畜牧兽医学报2017，Vol.48Issue(1)：23-30,8.DOI:10.11843/j.issn.0366-6964.2017.01.003
鸡性成熟启动前后下丘脑microRNA表达谱分析
Analysis of Hypothalamic microRNA Expression Profile during Chicken Puberty Onset
摘要
Abstract
The objective of this study was to investigate whether microRNAs play roles in puberty onset of the chicken.The Solexa deep sequencing was performed to analyze the miRNA expression profiles in the hypothalamus of hens before puberty onset (BPO) and after puberty onset (APO).The target genes prediction and functional analysis were also carried out through bioinformatics method.374 conserved and 46 novel miRNAs were identified in the chicken hypothalamus.144 conserved miRNAs were differentially expressed (reads＞10,P＜0.05) from BPO to APO transition.2 013 putative genes were predicted as the targets of the 15 differentially expressed miRNAs (| log2 (fold-change) | ＞ 2.0,P ＜ 0.01).Functional analysis suggested that these 15 miRNAs played important roles in transcriptional regulation and signal transduction during puberty onset.The results suggest that miRNA as novel partner involve in chicken puberty onset.Considering the characteristics of miRNA functional conservation,the results will provide new theoretical base for studying the molecular regulation mechanism of animals puberty onset.关键词
鸡/性成熟启动/下丘脑/miRNA表达谱Key words
chicken/puberty onset/hypothalamus/miRNA expression profile分类
农业科技引用本文复制引用
韩威,邹剑敏,盛中伟,朱云芬,李国辉,王洪志,张会永,殷建玫,苏一军,王克华..鸡性成熟启动前后下丘脑microRNA表达谱分析[J].畜牧兽医学报,2017,48(1):23-30,8.基金项目
国家自然科学基金(31201799) （31201799）
江苏省农业科技创新项目(CX[15]1009) （CX[15]1009）
