畜牧兽医学报2017，Vol.48Issue(1)：31-38,8.DOI:10.11843/j.issn.0366-6964.2017.01.004
山羊FGF21基因克隆及其在肌内脂肪细胞中的表达模式研究
Cloning of Goat FGF21 Gene and Its Expression Pattern in Intramuscular Adipocyte
摘要
Abstract
This study was conducted to obtain the sequence of goat FGF21 gene,elucidate the expression characteristics of FGF21 gene in different tissues,and detect the expression of FGF21 and FGFR during intramuscular preadipocyte differentiation.Jianzhou Big-eared goat was used as laboratory animal in this study.The goat intramuscular preadipocytes were obtained using colla genase Ⅱ.RT-PCR was used to clone FGF21 gene.Real-time quantitative PCR (qPCR) was performed to detect the expression of FGF21 in different tissues and the expression levels of FGF21 and its receptors during goat intramuscular preadipocyte differentiation.The full-length sequence of goat FGF21 gene was 666 bp including a complete 630 bp open reading frame (ORF),encoding 209 amino acids (Accession No.:KT288195).FGF21 gene was expressed in all examined tissues including heart,liver,spleen,lung,kidney,adipose tissue and longissimus dorsi,and enriched in liver and adipose tissue.During the differentiation of goat intramuscular preadipocyte,the expression pattern of FGF21 gene was the same as that of FGFR1 and FGFR2,peaked at 2nd day,extremely higher than other days (P＜0.01).The FGF21 mRNA was enriched in adipose tissue and peaked at 2nd day during goat intramuscular preadipocyte differentiation,indicating it might be a regulator in the early differentiation progress via FGFR1 or FGFR2.These results provide data for further elucidating the molecular mechanism of FGF21 gene in regulating goat IMF deposition.关键词
FGF21/FGFR/山羊/克隆/表达/肌内前体脂肪细胞Key words
FGF21/FGFR/goat/clone/expression/intramuscular preadipocytes分类
农业科技引用本文复制引用
李倩,林亚秋,朱江江,林森,张小玉,王永..山羊FGF21基因克隆及其在肌内脂肪细胞中的表达模式研究[J].畜牧兽医学报,2017,48(1):31-38,8.基金项目
四川省“十三五”畜禽育种攻关项目(2016NYZ0045) （2016NYZ0045）
四川省科技创新产业链示范工程重大项目(2014NZ0003) （2014NZ0003）
中央高校基本科研业务费专项资金项目(2016NZYQN36) （2016NZYQN36）
