畜牧兽医学报2017，Vol.48Issue(1)：39-51,13.DOI:10.11843/j.issn.0366-6964.2017.01.005
绵羊多羔主效基因FecB高通量检测方法的建立及应用
Establishment of High-throughput Molecular Detection Methods for Ovine High Fecundity Major Gene FecB and Their Application
摘要
Abstract
Based on the previous studies,the aim of this study was to further improve the efficiency and accuracy of genotyping.Two high-throughput genotyping methods (Taqman and SNaP-shot) for FecB detection in Chinese sheep breeds had been established in the present study.The results showed that the two methods not only reduced the cost but also saved the time and increased efficiency dramatically.The two methods had potential application value in large-scale molecular breeding introducing FecB.The detection results of 23 264 sheep including 10 Chinese indigenous sheep breeds,3 crossbred breeds and some hybridized sheep populations collected since 2003 showed that the allele B frequencies in Small Tail Han and Hu sheep were much higher than other breeds.The allele B frequency in Small Tail Han sheep was 0.432-0.833.The allele B frequency in Luxi Blackhead sheep had been improved (up to 0.432) during upgrading and selection stage,while decreased during intercross stage.These results can provide reference data for improving sheep fecundity in sheep breeding program by introducing FecB gene.关键词
FecB基因/产羔数/Taqman探针法/SNaPshot分型/等位基因频率/绵羊Key words
FecB gene/litter size/Taqman probe method/SNaPshot genotyping/allele frequency/sheep分类
农业科技引用本文复制引用
刘秋月,狄冉,曹晓涵,王翔宇,储明星,胡文萍,贺小云,潘章源,郭晓飞,冯涛,曹贵玲,黄冬维,贺建宁..绵羊多羔主效基因FecB高通量检测方法的建立及应用[J].畜牧兽医学报,2017,48(1):39-51,13.基金项目
国家自然科学基金项目(31472078 （）
31402041 （）
31572371 （）
31501941 （）
31501926) （）
国家肉羊产业技术体系专项(CARS-39) （CARS-39）
中央级公益性科研院所基本科研业务费专项(2013ywf-zd-1 （）
2015ywf-zd-2 （）
2015ywf-zd-8) （）
中国农业科学院科技创新工程(ASTIP-IAS13) （ASTIP-IAS13）
内蒙古自治区科技重大专项 （）
内蒙古自治区战略性新兴产业发展专项资金计划 （）
宁夏农林科学院科技创新先导资金项目(DWJLC-2016001) （DWJLC-2016001）
