畜牧兽医学报2017，Vol.48Issue(1)：52-59,8.DOI:10.11843/j.issn.0366-6964.2017.01.006
牦牛FKBP6基因启动子区克隆、鉴定与分析
Cloning and Identification of Promoter of Yak FKBP6 Gene
摘要
Abstract
The aim of this study was to understand 5'regulatory region and promoter region features of FKBP6 gene,to provide evidence for exploring the mechanisms of differential expression of FKBP6 gene in yak and yak-cattle testicular tissue.The sequence of 5'regulatory region of yak FKBP6 gene was obtained by cloning and sequencing,and was analyzed by bioinformatics methods.The core promoter region of FKBP6 gene was identified using Dual-luciferase assay system and the transcription factor binding sites related to spermatogenesis were predicted using bioinformatics software.The 5'regulatory region of yak FKBP6 gene was 1 354 bp in length by cloning,sequencing and splicing,which shared the similarity of 99.71％ with cattle.The 5'regulatory region sequence of yak FKBP6 gene contained several potential promoter regions,typical CAAT-Box and CpG island,but no TATA-Box.The core promoter region of FKBP6 gene was located in-263--167 nt of 5'regulatory region of FKBP6 gene by luciferase activity analysis and contained transcription factor binding sites related to spermatogenesis including CAAT-Box,E-Box,CTCF and CREB.The identification of the FKBP6 gene core promoter and the transcription factor binding sites provide a basis for further research on the regulation of FKBP6 gene expression in yak testis.关键词
牦牛/FKBP6/5'调控区/核心启动子Key words
yak/FKBP6/5'regulatory region/core promoter分类
农业科技引用本文复制引用
姚一龙,李伯江,李齐发..牦牛FKBP6基因启动子区克隆、鉴定与分析[J].畜牧兽医学报,2017,48(1):52-59,8.基金项目
国家自然科学基金(30500360) （30500360）
