畜牧兽医学报2017，Vol.48Issue(1)：99-107,9.DOI:10.11843/j.issn.0366-6964.2017.01.012
胰高血糖素样肽-2和二肽基肽酶Ⅳ抑制剂联合使用对断奶仔猪肠上皮细胞的影响
Effects of the Combination of Glucagon-like Peptide-2 and Dipeptidyl Peptidase-Ⅳ Inhibitors on Intestinal Epithelial Cells of Weaned Piglets in vitro
摘要
Abstract
This study was conducted to investigate the effects of the combination of Glucagon-like peptide-2 (GLP-2) and dipeptidyl peptidase-Ⅳ (DPP-Ⅳ) inhibitors with different concentrations on cell proliferation,metabolism and enzyme activity of primarily intestinal epithelial cells cultured in vitro from 28-day-old weaned piglets,and aimed to explore the method of promoting GLP-2 effects.The experiment,taking 28-day-old weaned piglets' intestinal epithelial cells as a model,studied the effects of DPP-Ⅳ inhibitors (KR62436) with different concentrations on the cell proliferation and metabolism.The 2 × 3 factorial design was adopted to study the effects of combination of GLP-2 (1×10-10,1×10-9 and 1×10-8 mol · L-1) and KR62436 (0 and 1×10-11 mol · L-1) on cell proliferation,metabolism and apoptosis.The results were as follows:there was no significant effect of DPP-Ⅳ with 1× 10-11 mol · L-1 on cell number,MTT OD,cell protein retention,total cell protein level,LDH activity,CK activity and Na+,K+-ATPase activity (P＞0.05).Compared with the control group,the DPP-Ⅳ group with 1 × 10-10 mol · L-1 showed lower MTT OD value (P＜0.05),and higher LDH and CK activity (P＜0.05).In the groups that treated with different concentrations of GLP-2 and low concentrations of KR62436,with the GLP-2 doses increasing,cell number,MTT OD value,cell protein retention,total cell protein level and Na+,K+-ATPase activity increased significantly (P＜0.05),and LDH and CK activity decreased significantly (P＜0.05).KR62436 could significantly promote GLP-2 effects.The results indicate that the low concentration DPP-Ⅳ inhibitors have no significant effect on cell growth,while the high concentration DPP-Ⅳ inhibitor have a negative effect on cell metabolism and integrity.Combination of GLP-2 and low concentrations DPP-Ⅳ inhibitors may have favorable effects on intestinal epithelial cell proliferation,metabolism and integrity than GLP-2 alone.关键词
胰高血糖素样肽-2/DPP-Ⅳ抑制剂/肠上皮细胞/增殖/完整性Key words
glucagon-like peptide-2/dipeptidyl peptidase-Ⅳ inhibitors/intestinal epithelial cell/proliferation/integrity分类
农业科技引用本文复制引用
贾刚,邓秋红,蒋荣川,赵华,陈小玲,刘光芒,王康宁..胰高血糖素样肽-2和二肽基肽酶Ⅳ抑制剂联合使用对断奶仔猪肠上皮细胞的影响[J].畜牧兽医学报,2017,48(1):99-107,9.基金项目
高等学校博士学科点专项科研基金(博导类)(20125103110011) （博导类）
四川省科技支撑计划(2013NZ0054) （2013NZ0054）
