畜牧兽医学报2017，Vol.48Issue(1)：116-123,8.DOI:10.11843/j.issn.0366-6964.2017.01.014
禽呼肠孤病毒感染对SPF鸡外周血淋巴细胞中天然免疫相关基因转录的影响
Effects of Avian Reovirus Infection on Transcription of Innate Immune Genes in Peripheral Blood Lymphocytes of SPF Chickens
摘要
Abstract
This study was aimed to assess the influences of avian reovirus (ARV) infection on expression of innate immune genes in peripheral blood lymphocytes of SPF chickens.The quantitative real-time PCR assays was performed to determine the transcriptional levels of TLR3,TLR7,TLR21,MDA5,IPS-1,IRF-3,IFN-α,IFN-β,IFN-γ,IFITM3,Mx1 and OASL in 0 h,12 h,1,2,3,5 and 7 days post infection (dpi) in peripheral blood lymphocytes of SPF chickens infected with ARV S1133.These results showed that the mRNA transcriptional levels of TLR3 and TLR21 were significantly reduced within 7 dpi (P＜0.05 or P＜0.01);the mRNA transcriptional levels of TLR7 and MDA5 were obviously promoted and specially reached the summit maximum values on 7 dpi and 1 dpi,respectively (P＜0.05 or P＜0.01).The mRNA level of IPS-1 was down-regulated within 7 dpi.Meanwhile,the mRNA level of IRF-3 was up-regulated during the whole transcrption course,and IFN-α,IFN-β and IFN-γ mRNA levels were significantly increased and reached the peak at 2-3 dpi.The mRNA levels of IFITM3,Mxl and OASL were significantly up-regulated and peaked at 3 dpi (P＜0.01).Therefore,ARV infection is able to modulate the increased mRNA transcription of TLR7,MDA5,IRF-3,IFN-α,IFN-β,IFN-γ,IFITM3,Mx1 and OASL,and reduce the mRNA transcription of TLR3,TLR21 and IPS-1,which would contribute to further understand ARV pathogenesis and host innate immune responses to ARV infection.关键词
禽呼肠孤病毒/天然免疫相关基因/SPF鸡外周血淋巴细胞Key words
avian reovirus/innate immune genes/peripheral blood lymphocyte of SPF chickens分类
农业科技引用本文复制引用
黄莉,张艳芳,王盛,曾婷婷,谢芝勋,蓝元喜,谢丽基,邓显文,范晴,罗思思,谢志勤,黄娇玲..禽呼肠孤病毒感染对SPF鸡外周血淋巴细胞中天然免疫相关基因转录的影响[J].畜牧兽医学报,2017,48(1):116-123,8.基金项目
广西自然科学基金(2014GXNSFCA118006) （2014GXNSFCA118006）
广西水产畜牧兽医局科技项目(桂渔牧科201452003) （桂渔牧科201452003）
国家自然科学基金(31660715) （31660715）
国家“万人计划”领军人才专项(2016-37) （2016-37）
广西兽医生物技术室开放基金(14-045-31-A-2) （14-045-31-A-2）
