畜牧兽医学报2017，Vol.48Issue(1)：124-131,8.DOI:10.11843/j.issn.0366-6964.2017.01.015
黄羽祖代公鸡精液作为禽白血病净化检测材料的应用研究
Applied Research of Yellow Feather Grandparent Roosters Semen as Test Sample in Avian Leukosis Eradication Program
摘要
Abstract
In order to investigate the role of semen of yellow feather grandparent roosters in avian leukosis (AL) eradication process,a,b,c three different breeds of yellow feather grandparent roosters were chosen to be sampled.ALV p27 antigen ELISA was used in direct detection of cloacal swabs,while anti-coagulated blood and semen sample were used to isolate and detect exogenous ALV based on DF-1 cells.Upon comparative analysis,the results showed that the virus could be isolated from semen,while the result of ALV p27 antigen ELISA S/P value of cloacal swabs and the virus isolation of plasma were negative.Three results couldn't completely correspond respectively.Individual AL deteetion of cloacal swabs,plasma or semen could lead to missed detection.Therefore in AL eradication of yellow feather breeds,semen as the test sample is feasible and should not be neglected,multiple samples should be considered in order to improve the detection rate and speed up the purification process.关键词
黄羽祖代公鸡/禽白血病/ALV p27/精液/净化Key words
yellow feather grandparent roosters/avian leukosis/ALV p27/semen/eradication分类
农业科技引用本文复制引用
饶明章,袁丽霞,赵子君,张杰,李锦群,廖明,曹伟胜..黄羽祖代公鸡精液作为禽白血病净化检测材料的应用研究[J].畜牧兽医学报,2017,48(1):124-131,8.基金项目
公益性行业(农业)科研专项(201203055) （农业）
国家肉鸡产业技术体系岗位科学家(CARS-42-G09) （CARS-42-G09）
广东省科技计划项目(2013B020224003 （）
2015A020209145) （）
广东省家禽产业技术体系(2016LM1114) （2016LM1114）
