畜牧兽医学报2017，Vol.48Issue(9)：1602-1610,9.DOI:10.11843/j.issn.0366-6964.2017.09.005
荣昌猪胸腺素β15A(TMSB15A)cDNA全长克隆、序列信息及组织表达分析
The Full-length Cloning,Sequence Information and Expression Analysis of cDNA of Thymosin β15A(TMSB15A) in Rongchang Pig
摘要
Abstract
The aim of the present study was to clone the full-length cDNA of TMSB15A,analyse the structure and function of TMSB15A gene and determine its expression profile in Rongchang pig.Using the RCAE (Rapid-amplification of cDNA ends)-PCR and RT-PCR(Real-time quantitative PCR),we cloned the full-length of TMSB15A mRNA and detected the expression profile of TMSB15A gene in Rongchang pig.The results showed that the TMSB15A mRNA of Rongchang pig was 654 bp in length.This sequence consisted of 138 bp of CDS,86 bp of 5'UTR and 430 bp of 3'UTR.The amino acid sequence deduced from TMSB15A encoded a putative protein consisting of 45 amino acids.Bioinformatics analysis indicated that TMSB15A protein had 10 negatively charged amino acids and 9 positively charged amino acids.The theoretical molecular weight of TMSB15A protein was 5.2 ku and the theoretical isoelectric point was 5.3.It was predicted that no hydrophobic amino acids,signal peptides or transmembrane domain were found in TMSB15A protein.TMSB15A protein might play physiological function in the cytoplasm.The tertiary structure of TMSB15A comprised α-helices and random coils.Rongchang pig TMSB15A had the greatest genetic relationship with TMSB15A of Homo sapiens,Nomascus leucogenys and Pan troglodytes (97.8％),followed by Camelus ferus and Oryctolagus cuniculus (93.3 ％),and the lowest relationship with Equus caballus(11.1 ％).The highest expression level of TMSB15A was in the spleen and thymus (P＜0.01).Medium expression levels were observed in lymph node and lung.The expression of TMSB15A in heart,liver,kidney and muscle was the lowest.These data provide a basis for investigating the functions of TMSB15A and will inform further research in Rongchang pig resistance breeding.关键词
昌猪/TMSB15A/RACE/基因克隆/序列分析/组织表达Key words
Rongchang pig/TMSB15A/RACE/gene clone/sequence analysis/tissues expression分类
农业科技引用本文复制引用
龙熙,蓝静,郭宗义,王金勇,赵久刚..荣昌猪胸腺素β15A(TMSB15A)cDNA全长克隆、序列信息及组织表达分析[J].畜牧兽医学报,2017,48(9):1602-1610,9.基金项目
重庆市农发资金项目(13405) （13405）
国家自然科学基金项目(31201770) （31201770）
基本科研业务费项目(2013cstc-jbky-00908) （2013cstc-jbky-00908）
重庆市畜牧科学院基本科研业务费(16435) （16435）
