畜牧兽医学报2017，Vol.48Issue(9)：1624-1634,11.DOI:10.11843/j.issn.0366-6964.2017.09.007
白来航蛋鸡产蛋前后肝转录组功能分析
Functional Analysis of Liver Transcriptome before and after White Leghorn Hen Begin Laying Eggs
摘要
Abstract
The objective of this study was to analyze the changes of the liver metabolic function and its effects on nutrients conversion and utility based on differentially expressed gene analysis in the liver.Full-sib White Leghorn layer hens for one week before and after first egg laying were chose for liver sampling.For analyzing and screening the important genes involved in the nutrient metabolic pathway in the liver,the transcriptome data was obtained by RNA-seq technology and bioinformatics analysis,and the gene expression differences was compared before and after the first egg laying.Two hundred and twenty-two differentially expressed genes with more than 2-fold changed were obtained by the transcriptomics sequencing (P＜0.05).GO enrichment analysis and KEGG pathway analysis of differentially expressed genes showed that differentially expressed genes obtained in this study mainly involved in lipid metabolism and tryptophan metabolism.In this study,there were some function-unknown differentially expressed genes were found,such as Ring finger protein 186 (RNF186).We obtained the differentially expressed genes in liver from White Leghorn hens before and after first eggs laying and analyzed the changes of metabolism pathway of nutrients in the liver.These results proved that the liver metabolism pathway of nutrients were changed by these differentially expressed genes and the changes might improve the nutrients utilization of the feed after first egg laying.关键词
蛋鸡/肝/转录组/脂类代谢Key words
layer hen/liver/transcriptome/lipid metabolism分类
农业科技引用本文复制引用
李慧锋,张臻,朱文进,张同玉,郭文婕,蔡永强,朱芷葳,张利环..白来航蛋鸡产蛋前后肝转录组功能分析[J].畜牧兽医学报,2017,48(9):1624-1634,11.基金项目
国家自然科学基金(31172203) （31172203）
山西省青年科技研究基金(2011021028-1) （2011021028-1）
山西省现代农业鸡产业技术体系 （）
