畜牧兽医学报2017，Vol.48Issue(9)：1635-1647,13.DOI:10.11843/j.issn.0366-6964.2017.09.008
水牛泌乳期与非泌乳期乳腺组织miRNAs表达谱鉴定及差异表达分析
Identify and Analyze the Expression Profile and Mechanism of Buffalo Mammary Gland miRNAs in the Lactation and Non-lactation Periods
摘要
Abstract
This study aimed to identify and analyze the expression patterns and mechanism of buffalo mammary gland miRNAs in the lactation and non-lactation periods.Buffalo mammary gland tissues in lactation and non-lactation periods were collected,their miRNAs expression profiles were analyzed by Solexa sequencing technology,pre-miRNAs,mature miRNAs and novel miRNAs were identified,respectively,the first preferred nucleotide and chromosome distribution were analyzed.The highly and differentially expressed miRNAs in the lactation and non-lactation periods were detected.The results showed that 2 miRNAs expression profiles from buffalo lactation and non-lactation mammary glands were constructed.12 768 110 and 12 569 467 high-quality reads between 18 and 31 nt were obtained seperately.Three hundred fifty-nine mature miRNAs and 363 pre-miRNAs belonged to 259 miRNAs families,and 5 buffalo-specific miRNAs were confirmed by sequencing.U was the most common nucleotide at the 5'end of 19 and 25 nt miRNAs.The bbu-let-7b,bbu-let-7a,miR-26a and miR-21 showed high expression in both periods.bbumiR-148a,143,200c,200a and bbu-let-7f were specificially highly expressed in non-lactation period,the bbu-miR-125b,29a and bbu-let-7c were specifically highly expressed in lactation period.The bbu-miR-148a,143,200a,141 and 30a-5p decreased the expression in the lactation period to less than 1/2 of the non-lactation period,bbu-miR-26a,29a,125b,99a and bbu let 7c were highly expressed in the lactation period more than or equal to 2 times of the lactation period.The result indicate that 2 miRNAs profiles from buffalo lactation and non-lactation mammary gland tissues were constructed.359 mature miRNAs and 363 pre-miRNAs belonging to 259 buffalo miRNAs families were identified.5 buffalo genome-specific miRNAs and 10 highly differentially expressed miRNAs were obtained,which lay the foundation for further elucidating the mechanism of key miRNAs in lactating buffalo.关键词
水牛/泌乳期和非泌乳期/乳腺组织/miRNA表达谱/信号通路Key words
buffalo/lactation and non-lactation periods/mammary gland tissue/miRNA expression pattern/signaling pathway分类
农业科技引用本文复制引用
蔡小艳,鲍正攀,古景开,邓凯,张晓溪,任艳萍,沈朋雷,石德顺,刘庆友..水牛泌乳期与非泌乳期乳腺组织miRNAs表达谱鉴定及差异表达分析[J].畜牧兽医学报,2017,48(9):1635-1647,13.基金项目
国家863重点项目课题(2013AA102504):广西科技厅项目(桂科合14125008-2-13 （2013AA102504）
桂科AD17129043 （）
桂科AA16380026) （）
