畜牧兽医学报2017，Vol.48Issue(9)：1648-1653,6.DOI:10.11843/j.issn.0366-6964.2017.09.009
抑制素对绵羊颗粒细胞雌激素和孕酮分泌及相关基因表达的影响
Effects of Inhibin on the Secretion of Estrogen, Progesterone and Expressions of Related Genes in Sheep Granulosa Cells
摘要
Abstract
The aim of this study was to investigate the effects of silencing the inhibin α-subunit (INHα) gene or treatment with inhibinA on the secretion of estrogen (E2),progesterone (P)and expressions of related genes in sheep granulosa cells (GCs),explore the role of inhibin in the secretion of E2 and P.GCs were isolated from antral follicles (3-7 mm) obtained from the ovaries of small-tailed Han sheep (1.0-1.5 years old).GCs were transfected with siRNA or treated with inhibinA (200 ng · mL-1).The concentrations of E2 and P were tested by ELISA.The mRNA levels of related-genes (CYP11,3β-HSD and CYP19) were analyzed by quantitative real-time PCR (qRT-PCR).The results showed that siRNA was transfected into GCs at 48 h and the silencing efficiency of INHα was 87％.Knockdown of INHα by siRNA decreased the concentrations of E2 and P at 48 h compared with the control (P＜0.05).Secretion of E2 and P were increased in the group treated with inhibinA (P＜0.05).Knockdown of INHα by siRNA decreased the mRNA expressions of 3β-HSD and CYP19,and increased the mRNA expression of CYP11 (P＜0.05).The mRNA expressions of 3β-HSD,CYP11 and CYP19 were increased in the group treated with inhibinA (P＜0.05).These results indicated that inhibin was a key regulator and involved in the process of follicular growth and ovulation by regulating the secretion of steroid hormone in sheep GCs.关键词
抑制素/雌激素/孕酮/绵羊/颗粒细胞/基因表达Key words
inhibin/estrogen/progesterone/sheep/granulosa cells/gene expression分类
农业科技引用本文复制引用
李婷,马爱团,刘月琴,高昆,张继伟,张英杰..抑制素对绵羊颗粒细胞雌激素和孕酮分泌及相关基因表达的影响[J].畜牧兽医学报,2017,48(9):1648-1653,6.基金项目
国家现代肉羊产业技术体系项目(CARS-39) （CARS-39）
