畜牧兽医学报2017，Vol.48Issue(9)：1654-1664,11.DOI:10.11843/j.issn.0366-6964.2017.09.010
非转移性黑色素瘤糖蛋白B对小鼠黑色素细胞色素生成的影响
Effects of GPNMB on Melanogenesis in Mouse Melanocytes
摘要
Abstract
This study aims to investigate whether GPNMB differentially expresses in the skin of mice with various hair colors and to determine its effect on the melanin synthesis.Three approximately 12-day-old healthy C57BL/6 mice were randomly selected from strains with black,brown and gray coat colors,respectively.Three pieces of dorsal skin tissue were collected from each mouse using a skin collector after shaving.Immunohistochemistry assay revealed that GPNMB was mainly located in the hair follicle matrix,the inner and outer root sheath,and the dermal papilla in the skin with various hair colors.The Western blot analysis and qRT-PCR assay showed that the expression level of GPNMB was the lowest in the skin of gray mice.Compared with the control group,relative levels of MC1R,β-catenin and SLC45A2 significantly increased in mouse melanocytes overexpressing GPNMB.we detected the levels of GPNMB and MC1R in mouse melanocytes which had been radiated by UVB.The results showed that UVB had an impact on the GPNMB and MC1R expression,and the level of GPNMB was various with different UVB radiation doses.The above results suggested that GPNMB was differentially expressed in the skin of mice with different hair colors,and it was involved in the formation of the hair color by regulating the melanin synthesis.关键词
GPNMB/UVB/黑色素细胞/黑色素Key words
GPNMB/UVB/melanocytes/melanin分类
生物科学引用本文复制引用
赵兵令,陈天直,杨玉静,于秀菊,杨林佩,董常生..非转移性黑色素瘤糖蛋白B对小鼠黑色素细胞色素生成的影响[J].畜牧兽医学报,2017,48(9):1654-1664,11.基金项目
国家高科技研究发展计划(863计划)(2013AA102506) （863计划）
国家公益性行业(农业)科研专项(201303119) （农业）
山西农业大学创新团队建设计划项目(CXTD201201) （CXTD201201）
