畜牧兽医学报2017，Vol.48Issue(9)：1711-1717,7.DOI:10.11843/j.issn.0366-6964.2017.09.016
2011-2016年云南新城疫病毒感染状况调查
Epidemiological Survey of Newcastle Disease Virus in Yunnan Province during 2011-2016
摘要
Abstract
Risk-based epidemiological survey was conducted in Yunan Province during 2011-2016 in order to better understand the epidemiological relations and their genetic diversity among the circulating Newcastle disease virus (NDV) in this region.A total of 2 565 swab samples were collected from 48 sampling units in Kunming,Yuxi,Dali,Lijiang,Pu'er and Qujing city.Results were as follows:Virus isolation and sequencing were used to determine the molecular characterization of the isolates.Fifty-two NDVs were isolated from samples collected from 17 live bird markets.The individual positive proportion of NDV was 2.03 ％,and the population positive proportion was 35.41％.Among 52 NDV isolates,17 NDVs isolated from 7 retail markets were proved to be virulent strains based on the sequence analysis of the Fusion gene.The individual positive proportion of virulent NDV was 0.66％,the population positive proportion of virulent NDV was 14.58％.Phylogenetic analysis revealed that all 52 NDV isolates were clustered into two classes,namely Class Ⅰ and Class Ⅱ.All 17 Class Ⅰ NDV isolates were belonged to genotype 3.Thirty-five Class Ⅱ NDV isolates comprised 4 different genotypes,namely genotype Ⅰ (2 isolates),Ⅱ (16 isolates),Ⅵ (13 isolates),Ⅶ (4 isolates).The genetic diversity of circulating NDVs in Yunnan province was proved,all Ⅵ NDV isolates were obtained from pigeon,and 4 genotype Ⅶ NDVs were isolates from chicken and goose.Two genotype Ⅶ h NDVs were first isolated from Yunnan in 2013 and 2016 respectively.Genotype Ⅶh NDVs has been endemic in some south-east Asian countries,such as Vietnam,Malaysia,Indonesia,etc.Wild birds may play the role for introduction from neighboring countries.So more active surveillance should be conducted to determine the distribution of the new virus and some control measures also need to be used to prevent the transmission of the new NDVs.关键词
新城疫病毒/流行病学调查/遗传演化分析Key words
Newcastle disease virus/epidemiological survey/phylogenetic analysis分类
农业科技引用本文复制引用
段博芳,相德才,刘华雷,张应国,王志亮,王静静,赵云玲,张凤安,吕嵘,吕艳,郑东霞,曾邦权,赵焕云..2011-2016年云南新城疫病毒感染状况调查[J].畜牧兽医学报,2017,48(9):1711-1717,7.基金项目
公益性行业(农业)科研专项(201303033) （农业）
