畜牧兽医学报2017，Vol.48Issue(9)：1724-1729,6.DOI:10.11843/j.issn.0366-6964.2017.09.018
犬细小病毒CPV-YH毒株的分离鉴定及其基因组变异分析
Isolation, Identification and Genomic Variation Analysis of Canine Parvovirus CPV-YH Strain
摘要
Abstract
To analyze genetic variation of canine parvovirus (CPV),a new virus strain was isolated from the feces of a dog infected by CPV.The virus strain,named as CPV-YH,was identified by red cell agglutination assay,morphology,artificial infection of Beagle dogs and molecular biology.The results showed that the isolate induced specific CPE in A-72 cells,and it could agglutinate porcine red blood cells.The virus were round or hexagonal by the electron microscope,and there was no capsule,with a diameter of about 20 nm.Animal regression test showed that the infected dogs had typical symptoms of CPV disease such as hemorrhagic enteritis,liver edema,pulmonary hyperaemia.The complete genome of CPV-YH was 4 921 nt and it shared the highest nucleotide identity (99％) with CPV-LZ2 isolates isolated from Lanzhou in 2011.The amino acid phylogenetic tree of VP2 showed that CPV-YH isolate,2013-BJ-P27 (China) and UY306 (Uruguay) isolates were on the same small branch.Compared with the CPV strains on GenBank,the homology of VP2 gene nucleotide and amino acid showed 98％-99.4％ and 97.1％-99.5％ identities,respectively.In conclusion,one CPV mutant strain was successfully isolated,and these data would provide valuable theoretical basis for the epidemic situation and prevention and control of CPV.关键词
犬细小病毒/分离鉴定/序列分析Key words
canine parvovirus/isolation and identification/sequence analysis分类
农业科技引用本文复制引用
孙明,邓小雨,刘巧荣,马永缨,张丽,卢会英,刘伯华,杨欣艳,陈西钊..犬细小病毒CPV-YH毒株的分离鉴定及其基因组变异分析[J].畜牧兽医学报,2017,48(9):1724-1729,6.基金项目
“十三五”国家重点研究计划(2016YFD0501006) （2016YFD0501006）
