中国蔬菜Issue(10)：39-44,6.
利用改良SOE-PCR技术定点突变TuMV C4-VPg基因
Site-directed Mutagenesis of TuMV C4-VPg Gene by Optimized Splicing by Overlap Extension PCR
摘要
Abstract
Turnip mosaic virus(TuMV)was an important disease for Brassica rapa L. ssp. pekinensis production,and the TuMV VPg gene had played a vital role infecting production process.While,the pathogenicity of different TuMV strains was diverse.This experiment adopted improved splicing by overlap extension PCR(SOE-PCR)technology,took TuMV-C4 VPg gene sequence as template,carried out site-directed mutagenesis on 5 different single-nucleotides between TuMV C4-VPg and TuMV CDN1-VPg genes. The results indicated that 5 single point mutants of TuMV C4-VPg were obtained by the optimized SOE-PCR,i.e. TuMV C4-VPg- Ⅰ,TuMV C4-VPg- Ⅱ,TuMV C4-VPg- Ⅲ,TuMV C4-VPg- Ⅳ and TuMV C4-VPg- Ⅴ.关键词
白菜类蔬菜/芜菁花叶病毒/VPg基因/重叠延伸PCR技术/单点突变Key words
Brassica rapa L. ssp. pekinensis/Turnip mosaic virus(TuMV)/VPg gene/Splicing by overlap extension PCR(SOE-PCR)/Single point mutant引用本文复制引用
李国亮,孙日飞,钱伟,张淑江,李菲,章时蕃,张慧,谢露露,武剑,王晓武..利用改良SOE-PCR技术定点突变TuMV C4-VPg基因[J].中国蔬菜,2017,(10):39-44,6.基金项目
国家自然科学基金项目(31372069),"十二五"国家科技支撑计划项目(2013BAD01B04-03),农业部园艺作物生物学与种质创制重点实验室项目 （31372069）
