中国现代医学杂志2017，Vol.27Issue(20)：6-10,5.DOI:10.3969/j.issn.1005-8982.2017.20.002
芍药甘草附子汤对类风湿关节炎大鼠下丘脑室旁核nNOS、IL-1β及TNF-α表达的影响
Effect of PGAD on nNOS, IL-1β and TNF-α expressions in hypothalamic paraventricular nuclei of rats with rheumatoid arthtis
谢传美 1袁国华1
作者信息
- 1. 川北医学院附属医院风湿科,四川南充637000
- 折叠
摘要
Abstract
Objective To investigate the effect of Peony and Glycyrrhiza and Aconite Decoction (PGAD)on the expressions of neuronal nitric oxide synthase (nNOS),IL-1β and TNF-α in the hypothalamic paraventricular nuclei of the rats with rheumatoid arthritis.Methods Forty-eight healthy Wistar rats were randomly divided into control group,model group and PGAD-intervention group with 16 in each group.The arthritis rat model was induced by type Ⅱ collagenase and evaluated by arthritis score and paw swelling score.The arthritis rats in the PGAD intervention group were given 30 mg/kg PGAD by gavage.Immunofluorescence and Western blot were used to detect the nNOS,IL-1β and TNF-α expressions in the hypothalamic paraventricular nuclei.Results The arthritis score,the paw swelling score,and the expressions of nNOS,IL-13 and TNF-α in the hypothalamic paraventricular nuclei were significantly higher in the model and PGAD-intervention groups than in the control group (P ＜ 0.05).but significantly lower in the PGAD intervention group than in the model group (P＜ 0.05).Conclusions PGAD has therapeutic and protective effect on the rats with rheumatoid arthritis,which may be related to the inhibition of nNOS,IL-1β and TNF-α expressions in the hypothalamic paraventricular nuclei.关键词
芍药甘草附子汤/类风湿关节炎/下丘脑室旁核/一氧化氮合酶/白介素1β/肿瘤坏死因子αKey words
Peony and Glycyrrhiza and Aconite Decoction/rheumatoid arthritis/hypothalamic paraventricular nucleus/nNOS/IL-1β/TNF-α分类
医药卫生引用本文复制引用
谢传美,袁国华..芍药甘草附子汤对类风湿关节炎大鼠下丘脑室旁核nNOS、IL-1β及TNF-α表达的影响[J].中国现代医学杂志,2017,27(20):6-10,5.
