中国现代医学杂志2017，Vol.27Issue(20)：16-20,5.DOI:10.3969/j.issn.1005-8982.2017.20.004
不同比例激活剂对富血小板凝胶的生物学影响
Effect of different proportions of activators on biological functions of platelet-rich gel
摘要
Abstract
Objective To compare the effect of different proportions of activators on the biological function of platelet-rich gel and to reaserch the feasibility for the reconstruction of corneal stroma.Methods Rabbit blood was extracted and platelet-rieh plasma (PRP) was prepared by two-step eentrifugation.Platelet-rich gels were prepared by activation of PRP with thrombin activators in different proportions and divided into three groups.There were group 9∶1 (PRP:activators =9∶1),group 11∶1 (PRP∶activators =11∶1) and group 5∶1 (PRP∶ activators =5∶1).The rabbit keratoeytes were seperately eo-cuhured with the three platelet-rich gels and the morphological features were observed.CCK-8 assay was used to analyze the influences of composite growth factors released from the platelet-rich gel on the proliferation of rabbit keratocytes in the three gronps.The adhesive properties of the three platelet-rich gels were evaluated after adhesion to the double-layer porcine corneal stroma.Results The platelet-rich gel of the group 9∶1 had better effect on promoting cell proliferation than those of other two groups.HE staining displayed that only the platelet-rich gel prepared in the group 9∶1 formed an orderly network scaffold that could closely attach to the double-layer porcine corneal stroma.Conclusions The platelet-rich gel,prepared by combining PRP with activators in 9 ∶ 1 proportion,can effectively promote the proliferation of keratocytes,and has the nature of orderly network structure,effective adhesiveness and favorable biocompatibility.These properties indicate that the platelet-rich gel prepared by combining PRP with activators in 9 ∶ 1 proportion is an excellent scaffold for the reconstruction of corneal stroma.关键词
激活剂/富血小板凝胶/角膜基质Key words
activator/platelet-rich gel/corneal stroma分类
医药卫生引用本文复制引用
戴静,陈建苏,招志毅..不同比例激活剂对富血小板凝胶的生物学影响[J].中国现代医学杂志,2017,27(20):16-20,5.基金项目
国家自然科学基金(No:30973244) （No:30973244）
