中国现代医学杂志2017，Vol.27Issue(20)：21-25,5.DOI:10.3969/j.issn.1005-8982.2017.20.005
DMT1在人胎盘绒毛膜癌细胞中表达的实验研究
Expression of divalent metal transporter 1 in BeWo cells
摘要
Abstract
Objective To further determine the positioning and the mRNA expression changes of divalent metal transporter 1 (DMT1) under iron deficiency conditions in human placental choriocarcinoma BeWo cells.Methods Adopting immunocytochemical technique,the localization of DMT1 in BeWo cells was detected.RNA of the cells was extracted,and detected the changes of DMT1 +IRE mRNA and DMTI-IRE mRNA using qRT-PCR.Results DMT1 was positively expressed in BeWo cells,and distributed in cytoplasm and membrane.The expressions of DMT1 +IRE and DMT1-IRE mRNAs raised with the increase of drug concentration and time of iron deficiency treatment.The relative expression level of DMT1+IRE mRNA was significantly higher than that of DMT1-IRE mRNA.Conclusions DMT1 is abundantly expressed in the cytoplasm and cell membrane of BeWo cells and regulated by the body's iron levels,suggesting that DMT1 plays an important role during the iron transfer process in the placenta,which provides an experimental basis for the study of molecular mechanisn of placental iron transport.关键词
铁缺乏/铁转运/BeWo细胞/DMT1Key words
iron deficiency/iron transport/BeWo cell/divalent metal transporter 1分类
医药卫生引用本文复制引用
曹晓晓,李燕琴..DMT1在人胎盘绒毛膜癌细胞中表达的实验研究[J].中国现代医学杂志,2017,27(20):21-25,5.基金项目
国家自然科学基金(No:30800909) （No:30800909）
