中国现代医学杂志2017，Vol.27Issue(20)：26-33,8.DOI:10.3969/j.issn.1005-8982.2017.20.006
IEX-1蛋白在胃癌中的表达及其意义
Expression of IEX-1 protein in gastric carcinoma and its effect on apoptosis and proliferation of human gastric cancer cells
摘要
Abstract
Objective To reveal the expression of IEX-1 protein in gastric cancer tissues and cell lines,and investigate the effect of IEX-1 on the proliferation and apoptosis of human gastric cancer cells.Methods Immunohistochemistry was used to detect IEX-1 protein expression in 100 samples of gastric macosal biopsy.IEX-1 gene and protein expressions were detected by fluorescence qRT-PCR and Western Blot respectively in normal gastric mucosa GES-1 cells and gastric cancer MKN28 cells.pcDNA3-FLAG-IEX-1 plasmid and pLL3.7-si-IEX-1 plasmid were transfected into MKN28 cells respectively,and then cell cycle,apoptosis and proliferation were detected by flow cytometry and MTT assay.Results The expression of TEX-1 protein in the gastric cancer tissues was higher than that in the normal gastric tissues and the chronic gastritis tissues (P＜0.05),and the expression level of IEX-1 was gradually increased with the progress of the disease.The expressions of IEX-1 gene and protein in the gastric cancer MKN28 ceils were higher than those in the normal gastric mucosal GES-1 cells (P＜0.05).Compared with the control group,the increased expression of IEX-1 protein could increase the proportion of the cells in S phase,decrease the proportion of the cells in G2 phase,increase the rate of cell proliferation,and decrease apoptosis (P ＜ 0.05).After the expression of IEX-1 protein was interfered,the percentage of G1 phase cells increased,that of G2 phase cells decreased,the rate of cell proliferation decreased,apoptosis increased (P＜ 0.05).Conclusions IEX-1 protein is highly expressed in gastric cancer tissues.Up-regulation of IEX-1 protein expression can promote the proliferation of human gastric cancer cells and play an anti-apoptotic role.Interfering with the expression of IEX-1 protein could block cell cycle progression and induce apoptosis.关键词
胃癌/即刻早期反应X-1/增殖/凋亡Key words
gastric cancer/IEX-1/proliferation/apoptosis分类
医药卫生引用本文复制引用
毛山山,程小珍,崔荣花,元建华,王美清..IEX-1蛋白在胃癌中的表达及其意义[J].中国现代医学杂志,2017,27(20):26-33,8.基金项目
2013年海南省海口市重点科技计划项目(No:2013-70) （No:2013-70）
