中国现代医学杂志2017，Vol.27Issue(20)：34-39,6.DOI:10.3969/j.issn.1005-8982.2017.20.007
东莞地区临床分离耐碳青霉烯的鲍曼不动杆菌的耐药特点及其机制研究
Study on genotyping of beta-lactamase in Carbapenem-resistant Acinetobacter baumannii separated in Dongguan area
郭主声 1林偲思 1朱学海 1张丽 1张丽华 1胡继华 2周谋清 2阎红霞 3郭少卿4
作者信息
- 1. 中山大学附属东莞东华医院检验科,广东东莞523110
- 2. 中山大学附属东莞东华医院感染科,广东东莞523110
- 3. 中山大学附属东莞东华医院呼吸科,广东东莞523110
- 4. 中山大学附属东莞东华医院急诊科,广东东莞523110
- 折叠
摘要
Abstract
Objective To analyze clinical distribution and drug resistance of Acinetobacter baumannii isolated in Tungwah Hospital,to learn [β-1actamase gene of Acinetobacter baumannii resistant to Carbapenem so as to provide the basis for clinical use of drugs and prevention of nosocomial infections.Methods Thirty strains of non-repetitive Acinetobacter baumannii resistant to Carbapenem which were isolated in Tungwah Hospital from January 2011 to December 2013 were collected.Agar dilution method was used to detect the minimal inhibitory concentrations (MIC) of 11 kinds of antimicrobial agents.Pidemiological analysis was conducted.Carbapenemase was screened by modified Hodge test.The metalloenzyme phenotype and AmpC enzyme phenotype of the tested strains were detected by EDTA-disk synergy test and three-dimensional test respectively.PCR amplification of OXA-23,OXA-24 and pulsed field gel electrophoresis (PFGE) homology analysis were used to analyze the moleculer type and genetic relationship between the resistant strains.Results In the 14 antimicrobial agents with monitored MIC,the resistance rate of 13 species was over 60％,only that of SCF was lower than 50％.Totally 30 strains of Carbapenem-resistant Acinetobacter baumannii were screened out,of which the metalloenzyme phenotype and gene were both negative,21 strains had AmpC enzyme and 24 strains showed positive modified Hodge test;among which OXA-23 gene was amplified in 26 strains while OXA-24 gene was not detected.Conclusions The resistance of Aeinetobaeter baumannii isolated in clinic of Dongguan area is very severe.OXA-23 gene is the main carbapenemase gene,and Carbapenemresistant strains are prevalent in this area.关键词
鲍曼不动杆菌/耐碳青霉烯酶/药敏实验/耐药基因Key words
Acinetobacter baumannii/resistance to carbapenemase/drug sensitive test/drug resistance gene分类
医药卫生引用本文复制引用
郭主声,林偲思,朱学海,张丽,张丽华,胡继华,周谋清,阎红霞,郭少卿..东莞地区临床分离耐碳青霉烯的鲍曼不动杆菌的耐药特点及其机制研究[J].中国现代医学杂志,2017,27(20):34-39,6.
