摘要
Abstract
Objective To explore the expressions of hypoxia inducible factor 1 (HIF-1α) and Rac1 proteins and their clinical significance in hepatocellular carcinoma.Methods Immunohistochemistry streptavidinperoxidase (SP) method was used to detect the expressions of HIF-1αand Racl proteins in 58 cases of hepatocellular carcinoma,42 cases of hepatocirrhosis and 20 cases of normal liver tissues.The findings were analyzed on basis of follow-up information.Results The cellular staining pattern for HIF-1α was predominantly in the nuclei or cytoplasm,while Racl was positively expressed in the cytoplasm.The positive rates of HIF-1α in the normal liver tissues,the hepatocirrhosis tissue and the hepatocellular carcinoma were 0.0％ (0/20),45.2％ (19/42) and 81.0％ (47/58) respectively,and there were significant differences among the three groups (P ＜ 0.05).The expression of HIF-1α protein in the hepatocellular carcinoma was closely correlated with the histological grade,TNM grade and lymphatic metastasis (P＜ 0.05).The positive rates of Rac1 in the normal liver tissues,the hepatocirrhosis tissue and the hepatocellular carcinoma were 0.0 (0/20),40.5％ (17/42) and 77.6％ (45/58) respectively,and there were significant differences among the groups (P＜ 0.05).The expression of Racl protein in the hepatocellular carcinoma was closely correlated with the histological grade,TNM grade and lymphatic metastasis (P＜ 0.05).Spearman rank correlation analysis revealed a positive correlation between HIF-1α and Rac1 expressions in the hepatocellular carcinoma (P＜ 0.05).Conclusions The expressions of HIF-1α and Rac1 are related to the canceration and progress of hepatocellular carcinoma,their united application can be used to determine the degree and prognosis of hepatocellular carcinoma.关键词
原发性肝癌/缺氧诱导因子-1α/Ras相关的C3肉毒底物1/免疫组织化学法Key words
hepatocellular carcinoma/hypoxia inducible factor-1α/Ras-related C3 botulinum toxin substrate 1/immunohistochemistry分类
医药卫生
