吉林大学学报（医学版）2017，Vol.43Issue(5)：857-861,后插1,6.DOI:10.13481/j.1671-587x.20170501
丁苯酞对血管性痴呆大鼠海马CA1区脑源性神经营养因子表达的影响
Effect of 3-n-butylphthalide on brain-derived neurotrophic factor expression in hippocampal CA1 region of vascular dementia rats
摘要
Abstract
Objective:To study the effect of 3-n-butylphthalide (NBP) on the expression of brain-derived neurotrophic factor (BDNF) in the hippocampal CA1 region of the vascular dementia (VD) rats,and to explore its protective effect on VD.Methods:Eighty healthy Wistar rats were equally and randomly assigned to sham operation group,NBP control group (sham operation + NBP injection),VD group (VD models),NBP treatment group (VD models + NBP injection) (n=20).Each group was divided into four subgroups (n =5):1,2,4,and 8 weeks after operation groups.The VD rat models were established by using permanent bilateral common carotid artery ligation.After consciousness,the rats in NBP treatment group and NBP control group were intraperitoneally injected with 5 mg · kg-1 · d-1 NBP for consecutive 7 d.The rats in VD and sham operation groups were intraperitoneally injected with 0.2 mL · d-1 saline for consecutive 7 d.At 1,2,4,and 8 weeks after operation,the rats in each group were decapitated.The brains were obtained,and then the hippoeampus tissues were isolated.The BDNF expression levels in the hippocampal CA1 region were determined using real-time quantitative PCR and immunohistochemistry methods.Results:At 2,4,and 8 weeks after s operation,the expression levels of BDNF mRNA in the hippocampus of the rats in VD group were significantly higher those in sham operation group (P＜ 0.05);at 4 and 8 weeks after operation,the expression levels of BDNF mRNA in the hippocampus of the rats in NBP treatment groups were significantly higher than that in VD group (P＜ 0.05).The immunohistochemistry results showed that the expression level of BDNF protein in the hippocampal CA1 region of the rats in VD group was higher than that in sham operation group at 4 weeks after operation (P＜ 0.05);the expression level of BDNF protein in the hippocampal CA1 region of the rats in NBP treatment group was higher than that in VD group at 8 weeks after operation (P＜0.05).Conclusion:The BDNF expression is increased in the hippocampal CA1 region of the rats with VD after the neurons were injured by ischemia.NBP can increase the BDNF expression level in the hippocampal CA1 region of the VD rats and protect the nerves.关键词
血管性痴呆/海马/脑源性神经营养因子/丁苯酞Key words
vascular dementia/hippocampus/brain-derived neurotrophic factor/3-n-butylphthalide分类
医药卫生引用本文复制引用
毛西京,王敏,于挺敏,姚刚..丁苯酞对血管性痴呆大鼠海马CA1区脑源性神经营养因子表达的影响[J].吉林大学学报（医学版）,2017,43(5):857-861,后插1,6.基金项目
国家自然科学基金资助课题(31372267,81500953) （31372267,81500953）
吉林省科技厅科技发展计划项目资助课题(20150520143JH) （20150520143JH）
