吉林大学学报（医学版）2017，Vol.43Issue(5)：862-866,5.DOI:10.13481/j.1671-587x.20170502
白细胞介素37对宫颈癌HeLa细胞顺铂化疗敏感性的增强作用
Enhancement of IL-37 in chemosensitivity of cervical cancer HeLa cells to cisplatin
摘要
Abstract
Objective:To transfer the interleukin 37 (IL-37) gene to cervical cancer Hela cells,and to explore the killing effect of IL-37 on the HeLa cells and its enhancement in the chemotherapy sensitivity of HeLa cells.Methods:The pIRES2-EGFP (NC group) and pIRES2-EGFP/IL-37 (IL-37 group) plasmids were transfected into the HeLa cells.Q-PCR and Western blotting methods were used to detect the expression levels of IL-37 mRNA and protein.The activities of HeLa cells in NC group,IL-37 group,DDP group and IL-37+DDP group were detected by CCK8 method,and the inhibitory rates of cells were calculated.The gene expressions of signal transducer and activator of transcription 3 (STAT3) and Cyclin D1 were detected by RT-PCR method.Results:Compared with NC group,the expression levels of IL-37 mRNA and protein in IL-37 group were significantly increased (P＜0.01).The activities of HeLa cells in DDP (5-15 mg · L-1) groups were inhibited after administration for 24-72 h (P＜ 0.01);the inhibitory rates in IL-37 + DDP group were higher than those in DDP group within 48 h after administration (P＜0.05).Compared with IL-37 group,the inhibitory rates in IL-37+DDP group was increased with 96 h after administration (P＜0.05).Compared with NC group,the expression levels of STAT3 and Cyclin D1 mRNA in IL-37 group were significantly decreased (P＜0.01).Conclusion:The over-expression of IL-37 can inhibit the proliferation of cervical cancer cells and enhance the effect of DDP on the chemotherapy of cervical cancer cells which may be related to the down-regulation of the expressions of STAT3 and Cyclin D1 by IL-37.关键词
宫颈肿瘤/白细胞介素37/顺铂/化疗敏感性Key words
cervical neoplasms/interleukin 37/cisplatin/chemotherapy sensitivity分类
医药卫生引用本文复制引用
邓子亮,王森,黎鹏,解奎龙,李淑贤,周世雄,贺晓舟,陈东,郭洪胜..白细胞介素37对宫颈癌HeLa细胞顺铂化疗敏感性的增强作用[J].吉林大学学报（医学版）,2017,43(5):862-866,5.基金项目
国家自然科学基金资助课题(81302244) （81302244）
广东省科技厅科技发展计划项目资助课题(2016A020215147,2013B021800062) （2016A020215147,2013B021800062）
广东省卫计委医学科研基金项目资助课题(A2016208,B2013293) （A2016208,B2013293）
广东省东莞市科技局社会发展重点基金项目资助课题(2013108101051) （2013108101051）
广东医科大学科研基金资助课题(M2016028) （M2016028）
广东省湛江市科技局科技计划项目资助课题(2013B01092) （2013B01092）
