吉林大学学报（医学版）2017，Vol.43Issue(5)：887-892,6.DOI:10.13481/j.1671-587x.20170506
沉默Smad4基因对乳腺癌MCF-7细胞增殖和凋亡的影响
Influence of silencing Smad4 gene in proliferation and apoptosis of breast carcinoma MCF-7 cells
摘要
Abstract
Objective:To study the effects of down-regulated Smad4 expression on the proliferation and apoptosis of breast carcinoma MCF-7 cells,and to explore their mechanisms.Methods:The human breast carcinoma MCF-7 cells and MDA-MB-231 cells were cultured in vitro.RT-PCR method was used to detect the expression levels of Smad4 mRNA in MCF-7 cells and MDA-MB-231 cells.The Smad4-shRNA plasmid and Scramble-shRNA plasmid were respectively stably transfected into the MCF-7 cells with high expression of Smad4.The experiment was divided into non-transfected MCF-7 cells (normal control) group,Smad4 gene silencing group,Scramble(negative control) group.The proliferation abilities of the cells in various groups were detected by CCK-8 method.The apoptotic rates of the cells in various groups were detected by flow cytometry.Real-time PCR method wasused to detect the mRNA expression levels of the proliferation-related genes CDKN1A,CDK1 and CDK2 and the apoptosisrelated genes Suvivin,bcl-2,caspase 3 and caspase 9.Results:The proliferation abilities of cells had no statistical significance between various groups (P＞0.05).The mRNA expression levels of CDKN1A,CDK1 and CDK2 in the cells had no statistical significance between various groups (P＞0.05).Compared with normal control group and negative control group,the apoptotic rate of the cells in Smad4 gene silencing group was significantly decreased (P＜0.01),the expression levels of Suvivin and bcl-2 mRNA in Smad4 gene silencing group were significantly increased (P＜0.01),and the mRNA expression levels of caspase 3 and caspase 9 in Smad4 gene silencing group were significantly decreased (P＜0.05).Conclusion:Smad4 could induce the apoptosis of MCF-7 cells by downregulating the expressions of Suvivin and bcl-2 and up-regulating the expressions of caspase 3 and caspase 9.关键词
Smad4/MCF-7细胞/细胞增殖/细胞凋亡Key words
Smad4/MCF-7 cells/cell proliferation/apoptosis分类
医药卫生引用本文复制引用
刘楠楠,李玉林,李荣贵,孙立伟,刘学娟..沉默Smad4基因对乳腺癌MCF-7细胞增殖和凋亡的影响[J].吉林大学学报（医学版）,2017,43(5):887-892,6.基金项目
国家科技部支撑计划项目资助课题(2012BAI19B05) （2012BAI19B05）
