吉林大学学报（医学版）2017，Vol.43Issue(5)：910-917,后插4,9.DOI:10.13481/j.1671-587x.20170510
HPK1过表达对乳腺癌MCF-7和MDA-MB-231细胞增殖和凋亡的影响及其机制
Influence of HPK1 overexpression in proliferation and apoptosis of breast cancer MCF-7 and MDA-MB-231 cells and its mechanism
摘要
Abstract
Objective:To investigate the effects of hematopoietic progenitor kinase 1 (HPK1) overexpression by construction of lentiviral vector on the proliferation and apoptosis of breast cancer MCF-7 and MDA-MB-231 cells,and to elucidate its possible mechanism.Methods:The cells were infected with the lentivirus overxpressing HPK1,and the MCF-7-HPK1 and MDA-MB-231-HPK1 cell lines were stably expressed HPK1;each cell line was divided into three experimental groups:blank group (untreated),control group (empty vector) and HPK1-overexpression group.The expression levels of HPK1 mRNA and protein in breast cancer cells in each group were detected by RTPCR and Western blotting methods,respectively.The cell proliferation rate was detected by MTT assay.The cell cycle and apoptotic rate were detected by flow cytometry.Transwell assay was used to analyze the cell migration ability.Western blotting method was used to measure the expression levels of caspase 3,PTEN,MMP-9,MMP-2,Ki-67and HPK1 proteins.Results:Compared with blank groups and control groups,the expression levels of HPK1 mRNA and protein in the both cell lines in HPK1 overexpression groups were significantly up-regulated (P＜0.05),the proliferation rates were significantly decreased (P＜0.05) and the apoptotic rates were significantly increased (P＜0.05),the number of cells crossing matrigel was significantly reduced (P＜0.05),the cell cycle of MCF-7 was blocked in G1 phase (P＜0.05),the expression levels of caspase 3 and PTEN proteins in HPK1 overexpression group were significantly increased (P＜0.05),and the expression levels of MMP-2 and MMP-9 proteins were significantly decreased (P＜0.05).Conclusion:HPK1 overexpression can inhibit the proliferation and migration of MCF-7 and MDA-MB-231 cells and induce apoptosis,which may be related to the up-regulation of caspase 3 and PTEN and down-regulation of MMP-9,MMP-2 and Ki-67.关键词
造血祖细胞激酶1/乳腺癌细胞株/细胞增殖/细胞凋亡Key words
hematopoietic progenitor kinase 1/breast cell lines/cell proliferation/apoptosis分类
医药卫生引用本文复制引用
王娇娇,范智蕊,李砺锋,丁显飞,周学良,赵杰,王留兴..HPK1过表达对乳腺癌MCF-7和MDA-MB-231细胞增殖和凋亡的影响及其机制[J].吉林大学学报（医学版）,2017,43(5):910-917,后插4,9.基金项目
河南省科技厅科技惠民计划专项基金资助课题(2013GS410101) （2013GS410101）
河南省科技厅重大科技专项基金资助课题(151100310800) （151100310800）
