吉林大学学报（医学版）2017，Vol.43Issue(5)：918-922,后插5,6.DOI:10.13481/j.1671-587x.20170511
PI3K/Akt信号通路介导莱菔硫烷预处理对大鼠供心冷缺血再灌注损伤的影响
Influence of sulforaphane preconditioning in myocardial cold ischemia-reperfusion injury through PI3K/Akt signaling pathway in rats
摘要
Abstract
Objective:To explore the effect of sulforaphane (SFN) preconditioning on the cold myocardial ischemia-reperfusion injury (IRI) in the rats through PI3K/Akt signaling pathway.Methods:Sixty-four health male Sprague-Dawley (SD) rats were randomly divided into cold IRI group,SFN group,LY (LY294002) + cold IRI group,and LY+SFN group (n=16).The allogeneic heterotopic heart transplantation model was established by donor heart into recipient abdomen.The myocardium tissue was taken 24 h after reperfusion for the detection of histological changes using HE staining.The expression levels of Akt,p-Akt,Bax and Bcl-2 proteins were detected by immunohistochemistry and Western boltting methods.Results:The morphological results showed that the myocardium tissue damage was serious in cold IRI group and LY+cold IRI group,it was light in SFN group;the myocardium tissue damage of the rats in SFN+ LY group was ranged between cold IRI group and SFN group.Compared with IRI group,the expression levels of p-Akt protein and Bcl-2 protein in SFN group were increased (P＜0.05),and the expression level of Bax protein was decreased (P＜0.05).After treatment of blockage LY294002,compared with LY-+-cold IRI group,the expression level of p-Akt protein in LY-+-SFN group was not statistically significant (P＞0.05),the expression level of Bcl2 protein was increased (P＜0.05),the expression levels of Bax protein was decreased),and the ratio of Bcl-2/Bax was also increased (P＜0.05).Conclusion:SFN may attenuate cold IRI of heart transplantation through PI3K/Akt signaling pathway in the rats.关键词
PI3K/Akt通路/莱菔硫烷/心脏移植/冷缺血再灌注损伤/细胞凋亡Key words
PI3K/Akt signaling pathway/sulforaphane/heart transplantation/cold ischemia reperfusion injury/apoptosis分类
医药卫生引用本文复制引用
伊雪,李占清,邬鹏宇,杨述亮,韩燕,杨学慧,张树波,徐涛,高毅哲,高木火..PI3K/Akt信号通路介导莱菔硫烷预处理对大鼠供心冷缺血再灌注损伤的影响[J].吉林大学学报（医学版）,2017,43(5):918-922,后插5,6.基金项目
福建省科技厅自然科学基金资助课题(2016D014) （2016D014）
福建省卫计委医学创新项目资助课题(2015-CXB-47) （2015-CXB-47）
