吉林大学学报（医学版）2017，Vol.43Issue(5)：928-931,后插5,5.DOI:10.13481/j.1671-587x.20170513
北五味子多糖对脂多糖诱导中性粒细胞炎症的抑制作用
Inhibitory effect of north-Schisandra chinensis polysaccharide on neutrophil inflammation induced by lipopolysaccharide
摘要
Abstract
Objective:To investigate the effect of Manchu medicine north-Schisandra chinensis polysaccharide (NSCP) on the human neutrophils treated by lipopolysaccharide (LPS) cultivated in vitro,and to elucidate its anti inflammatory mechanism.Methods:The neutrophilic inflammatory cell model was established with LPS.The experiment included control group,LPS group (1.0 mg · L-1) and NSCP group (1.25,2.50 and 5.00 g · L-1),the cells in NSCP group were first treated with LPS for 60 min,and then treated with different concentrations of NSCP.The levels of TNF-α in neutrophils were measured with ELISA and the apoptotic rates were detected by flow cytometry.Results:The level of TNF-a in LPS group was increased compared with control group (P＜0.05).The level of TNF-α in NSCP group was decreased compared with LPS group (P＜0.05).The apoptotic rate in LPS group was decreased compared with control group (P＜0.05);the apoptotic rates in NSCP group were increased with the increasing of time and dose,and the best effect was found 16 h after treatment with 5 g · L-1NSCP:the apoptotic rate in NSCP group was significantly increased compared with LPS group (P＜0.05).Conclusion:NSCP can perform the anti-inflammation effect through the suppression of LPS-induced TNF-α secretion in neutrophils and the promotion of neutrophils apoptosis.关键词
北五味子多糖/中性粒细胞/肿瘤坏死因子α/细胞凋亡Key words
north-Schisandra chinensis polysaccharide/neutrophil/tumor necrosis factor-a/cell apoptosis分类
医药卫生引用本文复制引用
孙晓红,李嘉仪,李洪涛,谢宇,孙新,孔繁利..北五味子多糖对脂多糖诱导中性粒细胞炎症的抑制作用[J].吉林大学学报（医学版）,2017,43(5):928-931,后插5,5.基金项目
吉林省科技厅科研基金资助课题(20130624003JC,20150312013ZG) （20130624003JC,20150312013ZG）
吉林省教育厅科学技术研究项目资助课题(2014201,201664) （2014201,201664）
北华大学省级大学生创新项目资助课题(2016-199100282) （2016-199100282）
北华大学博士启动基金资助课题(199500031) （199500031）
