成都中医药大学学报2017，Vol.40Issue(3)：45-48,4.DOI:10.13593/j.cnki.51-1501/r.2017.03.045
亚抑菌浓度隐丹参酮对表皮葡萄球菌生物膜形成的影响
Influence of Sub-inhibitory Concentration of Cryptotanshinone on Formation of Staphylococcus Epidermidis Biofilm
摘要
Abstract
Objective:To explore the influence of subinhibitory concentration of cryptotanshinone on the formation of staphylococcus epidermidis biofilm.Methods:Set the vancomycin as the drug contrast and detect the minimal inhibitory concentration (MIC) of cryptotanshinone and vancomycin by microdilution method;And semi-quantitative adherence assay 、XTT、scanning electron microscopy (SEM) and RT-PCR were used respectively for the determination of biofilm substrate quality,bacterial metabolism,microstructure and the expression of altE gene under the cryptotanshinone and vancomycin at 1/2 MIC level.Results:The MIC of cryptotanshinone and vancomycin were 2 μg/mL and 4 μg/mL respectively.When treated with 1/2 MIC of cryptotanshinone and vancomycin,the biofilm substrate quality,bacterial metabolism and expression of altE were inhibited.The inhibition of 1/2 MIC of cryptotanshinone was stronger than that of vancomycin,and the difference was statically significant (P ＜ 0.05).There was no biofilm formation treated with 1/2 MIC of cryptotanshinone,while the biofilm still formed with 1/2 MIC of vancomycin.Conclusion:Sub-inhibitory concentration of cryptotanshinone could inhibit the biofilm formation of staphylococcus epidermidis,which was stronger than the effects of subinhibitory concentration of vancomycin.关键词
表皮葡萄球菌/生物膜/亚抑菌浓度/隐丹参酮Key words
Staphylococcus epidermidis/Biofilms/Sub-MIC/Cryptotanshinone分类
医药卫生引用本文复制引用
易辉,易玉玲,祖瑞铃,李燕..亚抑菌浓度隐丹参酮对表皮葡萄球菌生物膜形成的影响[J].成都中医药大学学报,2017,40(3):45-48,4.基金项目
四川省科技厅应用基础研究项目(2015JY0159) （2015JY0159）
