一种生物刺激素对大豆根际土壤微生物群落的影响OA北大核心CSCDCSTPCD
Effect of A Kind of Biological Stimulant on Microbial Community in Rhizosphere Soil of Soybean
为研究生物刺激素(二苯基脲磺酸钙)对大豆根际土壤微生物群落的影响,采用中华费氏根瘤菌或不同浓度的生物刺激素对汾豆78进行浸种,田间条件下播种后在大豆花期时采集大豆根际土壤,并通过对比改良SDS法、TSAI法、生工试剂盒法和Mobile试剂盒法,选取效果最佳的Mobile试剂盒法进行土壤细菌DNA提取,对目的片段16S V3区进行特异性扩增,扩增产物用于后续高通量测序分析.结果表明:5个试验组与CK组相比,试验组Shannon数值下降.处理S2、S…查看全部>>
In this paper,field experiments were carried out to study the influence of biostimulant (Diphenylurea Sulfonic Calcium),developed by Shanxi University to the rhizosphere soil microbial community.Test set up five experimental groups,group S1 to S5,and one blank control group CK,then spray Chinese fisher Rhizobium or different concentrations of biostimulant to soybean seeds.Collected soybean rhizosphere soil during the flowering period,and compared the modifie…查看全部>>
袁鑫;王梦亮;王俊红
山西大学应用化学研究所,山西太原030006山西大学应用化学研究所,山西太原030006山西大学应用化学研究所,山西太原030006
农业科技
生物刺激素大豆根际微生物DNA提取高通量测序
BiostimulantSoybeanSoil rhizosphere microbialDNA extractionPyrosequencing
《大豆科学》 2017 (5)
751-760,10
林业公益性行业科研(201304326).
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