大豆科学2017，Vol.36Issue(5)：751-760,10.DOI:10.11861/j.issn.1000-9841.2017.05.0751
一种生物刺激素对大豆根际土壤微生物群落的影响
Effect of A Kind of Biological Stimulant on Microbial Community in Rhizosphere Soil of Soybean
摘要
Abstract
In this paper,field experiments were carried out to study the influence of biostimulant (Diphenylurea Sulfonic Calcium),developed by Shanxi University to the rhizosphere soil microbial community.Test set up five experimental groups,group S1 to S5,and one blank control group CK,then spray Chinese fisher Rhizobium or different concentrations of biostimulant to soybean seeds.Collected soybean rhizosphere soil during the flowering period,and compared the modified SDS method,TSAI method,Sangon magnetic bead kit and Mobile kit method,and selected the best Mobile Kit Method for soil bacterial DNA extraction.Amplified the fragment of 16S V3 region specifically,amplification products used for subsequent highthroughput sequencing analysis.The result showed that compared with the CK group,the Shannon values of the 5 experimental groups decreased.Compared with CK,S2,S3,S4 and S5 were found that the relative content of Proteobacteria,Actinomyces and Bacteroides were increased in the main phylum,and the relative contents of the phylum and the hard wall were reduced.At the genus level,it was also found that there was an increasing trend of functional bacteria in soybean growth and nitrogen fixation.Biostimulins made the diversity of soybean rhizosphere soil microorganism decreased,and could promote the growth of rhizobia,increase the content of rhizobia in rhizosphere soil microorganisms,at the same time,it could promote the number of functional bacteria in rhizosphere soil microbes,not only promote the soybean nitrogen fixation,but also promote the growth of soybean.关键词
生物刺激素/大豆/根际微生物/DNA提取/高通量测序Key words
Biostimulant/Soybean/Soil rhizosphere microbial/DNA extraction/Pyrosequencing分类
农业科技引用本文复制引用
袁鑫,王梦亮,王俊红..一种生物刺激素对大豆根际土壤微生物群落的影响[J].大豆科学,2017,36(5):751-760,10.基金项目
林业公益性行业科研(201304326). （201304326）
