大豆科学2017，Vol.36Issue(5)：761-767,7.DOI:10.11861/j.issn.1000-9841.2017.05.0761
大豆根瘤菌接种效应及竞争结瘤能力分析
Analysis of the Inoculation Effect of Soybean Rhizobia and the Competitive Nodulation Ability
摘要
Abstract
In order to screen and gain Bradyrhizobium japonicum strains that have high-competitive nodulation ability,in the mean time,be good for growth and quality of soybean,10 Bradyrhizobiumjaponicum strains which were being separating,identification and purification were used as materials to conduct field vaccination test with high-matching soybean.In the full-blossom period,testing the nitrogen fixation.At the same time,BOX-PCR was conducted with testing strains and new strains separated from the field,and BOX molecular fingerprint was compared,and calculated the rate of testing strains.Testing the mainly characters and yield components in the mature period.Result showed that:At the earlier stage,the leaves of inoculated soybean showed dark green and fresher,Bradyrhizobium japonicum could improve root nodules and the number of single nodules significantly.The number of single root nodules of I4 (the inoculation strain 112-1) was the most,compared with the non-inoculated control group of I0,32.95％ and the difference was significant.The dry weight of nodule treated with rhizobia was significantly lower than that of non-inoculated control.Among them,the inoculation treatment I4 had the highest dry weight.The inoculation treatment I4 had the highest nitrogenase activity,and there was no significant difference with the inoculation treatment I4.The incidence rate of the inoculation strain was between 10％ and 90％,and the rate of the tumor rate varied greatly,with the average tumor rate of 50％.I4 had a maximum rate of 90％,I6 (the inoculation strain 113-1)had the second-highest rate of 75％.The incidence of strain was a significant positively correlated with the number of single nodule (r =0.82 * *),there was a negative correlation between single tumor dry weight and rate,single root nodule and nitrogenase activity(r =-0.387,r =-0.50,r =-0.13).I6 had the highest plant height,followed by I4,the difference between the twotreatments was not significant,I2,I4 and I6 had relatively high single number of pod number and single grain number,and the difference between the three treatments was not significant,the hundred grain weight of soybean treated by I4 was significantly higher than that of other bacteria dispose.According to the effect of inoculation rhizobia on nitrogen fixation and yield of soybean nodules,the competitive nodules of the rhizobium strains additionally,this research screened out the high-value and higheffect strains 112-1and 113-1 that were appropriate for Harbin in Heilongjiang province.关键词
大豆根瘤菌/竞争结瘤/BOX-PCRKey words
Bradyrhizobium japonicum/Competitive nodulation/BOX-PCR分类
农业科技引用本文复制引用
王金生,丁宁,吴俊江,刘庆莉,张鑫..大豆根瘤菌接种效应及竞争结瘤能力分析[J].大豆科学,2017,36(5):761-767,7.基金项目
黑龙江省农科院院级科研项目专项资金(2017ZC06) （2017ZC06）
国家重点研发专项(2017YFD0101306). （2017YFD0101306）
