大豆科学2017，Vol.36Issue(5)：808-812,5.DOI:10.11861/j.issn.1000-9841.2017.05.0808
大豆花叶病毒侵染性克隆研究进展
Advances in Infectious Cloning of Soybean Mosaic Virus
摘要
Abstract
Soybean mosaic virus (SMV) is a complex and ever-changing virus that has a negative effect on the yield and quality of soybeans.Its serious pathogenicity is generally concerned by scholars associated with the field of soybean research.The SMV is mainly infected with soybean aphids.The research technique of soybean mosaic virus infection cloning was effective and widely adopted to study its pathogenic mechanism.This technique can further analyze the mechanism of the genes that cause the corresponding symptoms in the process of plant growth through the obtained infectious cDNA clones.At the same time,prepared monoclonal antibody can be used as the development of soybean mosaic virus detection kit probe material.As a basis for the study of SMV related fields,based on the relationship between soybean mosaic virus disease and SMV infection cloning technique.This paper briefly summarizes the construction strategy and influencing factors of SMV infectious clone,and summarizes the application of this technology in recent years,to provide new reference and thought for the control of soybean mosaic virus.关键词
大豆/大豆花叶病毒/侵染性克隆Key words
Soybean/Soybean mosaic virus/Infectious clones分类
农业科技引用本文复制引用
张恩柱,赵伟,张淋淋,韩庆玥,张俊华..大豆花叶病毒侵染性克隆研究进展[J].大豆科学,2017,36(5):808-812,5.基金项目
东北农业大学大学生创新基金(201610224147) （201610224147）
哈尔滨市应用技术研究与开发项目(2014AB6BN036) （2014AB6BN036）
中央引导地方科技发展专项(ZY16C06). （ZY16C06）
