南方医科大学学报2017，Vol.37Issue(10)：1322-1329,8.DOI:10.3969/j.issn.1673-4254.2017.10.07
Rictor/mTORC2调节小鼠睾丸血睾屏障与精子发生
Rictor/mTORC2 regulates blood-testis barrier and spermatogenesis in mice
摘要
Abstract
Objective To investigate the role of Rictor/mTORC2 in the formation of blood-testis barrier (BTB), testicular development, and spermatogenesis. Methods Amh-Cre-positive mice homozygous for rictor-loxP with Sertoli cell-specific deletion of rictor were obtained by cross breeding Amh-Cre mice with rictor-loxP mice. The histology of the reproductive organs,seminiferous tubules and epididymis of the transgenic mice was observed with HE staining.The cell subgroups of the germ cells in the seminiferous tubule were detected by flow cytometry with propidium iodide labeling.The expression levels of Ki-67 and separase were detected with immunofluorescence assay, and the expression levels of BTB-associated proteins were detected with immunofluorescence and Western blotting. Results Compared with the control (Amh-Cre-, rictorloxP/loxPor rictorloxP/-) mice, the mice with Sertoli cell-specific rictor deletion showed significantly decreased testicular weight and epididymis weight(P<0.05),significantly increased diploid cells(P<0.01),and decreased haploid cells(P<0.01)but comparable tetraploid cells and similar expression levels of Ki-67 and separase. The mice with rictor knockout also showed aberrant localization of BTB-associated proteins, which were scattered over the whole seminiferous epithelium, but the expression levels of the protein remained stable.Conclusion Rictor in testicular Sertoli cells is essential for maintaining BTB integrity and function and ensuring normal spermatogenesis in mice.关键词
血睾屏障/睾丸支持细胞/精子发生/哺乳动物雷帕霉素靶蛋白/RictorKey words
blood-testis barrier/testicular Sertoli cells/spermatogenesis/mammalian target of rapamycin/rictor引用本文复制引用
董合玲,吴洪渊,傅友,戴萌,白晓春,王红..Rictor/mTORC2调节小鼠睾丸血睾屏障与精子发生[J].南方医科大学学报,2017,37(10):1322-1329,8.基金项目
国家重大科学研究计划项目(科技部)(2013CB945203) （科技部）
国家自然科学基金(31401224) Supported by National Natural Science Foundation of China(31401224). （31401224）
