南方医科大学学报2017，Vol.37Issue(10)：1330-1336,7.DOI:10.3969/j.issn.1673-4254.2017.10.08
AQP-5对结直肠癌细胞生长的影响及机制
Role of aquaporin-5 in regulating colorectal cancer cell growth in vitro
摘要
Abstract
Objective Tostudy the role of aquaporin-5(AQP-5) in regulating the cell proliferation and apoptosis of human colorectal cancer cells and explore the possible mechanism.Methods A small interfering RNA(siRNA)targeting AQP-5 was used to suppress endogenous AQP-5 expression in the human colorectal cancer cell lines COLO 205 and SW480, and the transfection efficiency of AQP-5-siRNA was determined using immunofluorescence assay and PCR. The changes in the proliferation of the transfected cells was evaluated with MTT assay,and the cell apoptosis was analyzed using Annexin V-FITC/PI and TUNEL assays; the changes of Bax and Bcl-2 expressions in the cells were determined using RT-PCR and Western blotting.Results Transfection with AQP-5-siRNA resulted in a significant reduction(up to 90%)of AQP-5 expression in COLO 205 and SW480 cells.MTT assay showed that AQP-5-siRNA transfection significantly inhibited the cell proliferation compared NS-siRNA transfection (P<0.05). Flow cytometric analysis revealed significantly increased apoptotic rate of cells following AQP-5-siRNA transfection compared with NS-siRNA transfection(P<0.05). Real-time quantitative RT-PCR and Western blotting demonstrated that AQP-5-siRNA transfection significantly increased Bax and Bcl-2 expressions at both mRNA and protein levels in the cells.Conclusion AQP5-siRNA can promote apoptosis of colorectal cancer cells in vitro possibly in relation to its effects on Bax and Bcl expressions.关键词
结直肠癌/AQP-5/细胞凋亡/Bax/BclKey words
colorectal cancer/aquaporin-5/apoptosis/Bax/Bcl引用本文复制引用
陈硕,单涛,王丽,陈熹,崔熙娟,高显妮..AQP-5对结直肠癌细胞生长的影响及机制[J].南方医科大学学报,2017,37(10):1330-1336,7.基金项目
国家自然科学基金(81402583) （81402583）
陕西省自然科学基金(2014JQ4165) （2014JQ4165）
西安交通大学教育基金(xjj2014077) （xjj2014077）
西安交大二附院院基金(RC(XM)201402 （RC(XM）
YJ(QN)201521) Supported by National Natural Science Foundation of China(81402583). （QN）
