南方医科大学学报2017，Vol.37Issue(10)：1358-1363,6.DOI:10.3969/j.issn.1673-4254.2017.10.12
RhoA/Rho激酶通过上调大鼠脊髓背角中谷氨酰胺酶Ⅰ表达参与开胸术后慢性疼痛
RhoA/Rho-kinase contributes to chronic pain following thoracotomy by up-regulating glutaminase 1 expression in rat spinal dorsal cord
摘要
Abstract
Objective To investigate whether RhoA/Rho-kinase contributes to the occurrence of chronic post-thoracotomy pain (CPSP) by up-regulation of glutaminase 1 (GLS1) expression in the spinal dorsal cord. Methods Twenty-five male Sprague Dawley (SD) rats were divided into control group (n=5) and model group (n=20). The rats in the model group were randomized into two sub-groups(n=10)for observation on day 10 and day 21 after thoracotomy,and each group was further divided into CPSP and non-CPSP groups according to the behavioral test results.All the rats were sacrificed after behavioral test for examination of GLS1 and RhoA expressions in the spinal cord using Western blotting and RT-PCR.We also compared the effect of the Rho kinase inhibitor fasudil and saline,both injected intraperitoneally daily at 10 mg/kg for 7 consecutive days following thoracotomy,on CPSP and GLS1 expression in 30 male SD rats on day 21 after thoracotomy.Results Compared with the control group,the rats with CPSP showed significantly increased expressions of GLS1 and RhoA mRNA in the spinal cord on both day 10 and day 21 following thoracotomy(P<0.01),but the rats without CPSP did not show obvious changes in GLS1 and RhoA expressions.In fasudil-treated rats,the mechanical pain threshold was obviously increased and the expressions of GLS1 and RhoA were significantly reduced as compared with those in saline-treated rats(P<0.01).Conclusion RhoA plays an important role in the occurence of CPSP by up-regulating the expression of GLS1 in the spinal dorsal cord of rats.关键词
术后慢性疼痛/脊髓背角/谷氨酰胺酶Ⅰ/RhoA/Rho激酶Key words
chronic post-surgical pain/spinal dorsal cord/RhoA/Rho-kinase/glutaminase 1引用本文复制引用
刘紫嫣,王海棠,唐靖,秦再生..RhoA/Rho激酶通过上调大鼠脊髓背角中谷氨酰胺酶Ⅰ表达参与开胸术后慢性疼痛[J].南方医科大学学报,2017,37(10):1358-1363,6.基金项目
广东省医学科研基金(B2014255) （B2014255）
广东省科技计划项目(2014A020212394) （2014A020212394）
南方医院院长基金(2013C023,2014B018) （2013C023,2014B018）
南方医科大学科研启动计划(PY2013N043) （PY2013N043）
