南方医科大学学报2017，Vol.37Issue(10)：1370-1374,5.DOI:10.3969/j.issn.1673-4254.2017.10.14
稳定型心绞痛患者血浆差异蛋白的筛选及验证
Screening and verification of plasma biomarkers for stable angina pectoris:a differential proteomic analysis
摘要
Abstract
Objective To compare and analyze the differentially expressed plasma proteome between patients with stable angina pectoris(SAP)and healthy donors to identify the biomarkers for early diagnosis of SAP.Methods Plasma samples from 60 patients with SAP and 60 healthy controls were collected. Twenty samples (100 mL each) randomly selected from each group were pooled and after removing high-abundance proteins from the pooled plasma,two-dimensional gel electrophoresis (2DE) was performed to isolate the total proteins. The protein spots with more than 2-fold changes were selected after 2D analysis using software, and the differentially expressed proteins were identified by MALDI-TOF/TOF mass spectrometer. ELISA was performed to detect hemoglobin subunit delta(HBD)levels in 40 randomly selected samples from each group for verification of the results of 2DE. Results A total of 7 differentially expressed proteins were found in plasma samples from patients with SAP, including 3 up-regulated proteins (serum albumin, hemoglobin subunit alpha and hemoglobin subunit delta,) and 4 down-regulated ones (apolipoprotein L1, apolipoprotein C3, apolipoprotein E and complement C4-B). ELISA results showed that HBD level was increased in SAP plasma, which was consistent with the results of 2DE. Conclusion Patients with SAP have different plasma protein profiles from those of healthy controls, and HBD may serve as a potential specific biomarker for early diagnosis of SAP.关键词
稳定型心绞痛/血浆/生物标记物/蛋白质组学/双向凝胶电泳Key words
stable angina pectoris/plasma/biomarker/proteomics/two dimensional gel electrophoresis引用本文复制引用
沈安娜,胡水旺,黄敬,郑德仲,胡兆霆..稳定型心绞痛患者血浆差异蛋白的筛选及验证[J].南方医科大学学报,2017,37(10):1370-1374,5.基金项目
广东省科技计划项目(2013B021800309) （2013B021800309）
