南方医科大学学报2017，Vol.37Issue(10)：1400-1404,5.DOI:10.3969/j.issn.1673-4254.2017.10.20
眼球壁小梁网主要由平滑肌纤维构成且不存在内皮细胞
Histological structure of the trabecular meshwork in the eyeball: challenging the traditional concept and preliminary findings in rabbits,rats and mice
石赟 1周凡琦 1罗洲才 1陈英华 2陈煜 3董为人3
作者信息
- 1. 南方医科大学 基础医学院,广东 广州510515
- 2. 南方医科大学 组织学与胚胎学教研室,广东 广州510515
- 3. 南方医科大学 学实验教学管理中心//国家级医学基础实验教学示范中心//国家级医学形态学虚拟仿真实验教学中心,广东 广州510515
- 折叠
摘要
Abstract
Objective To verify that the trabecular meshwork (TM) in the wall of the eyeball consists of smooth muscle fibers instead of collagen fibers or endothelial cells. Methods Eighteen fresh eyeballs from 3 rabbits, 3 SD rats and 3 mice were sectioned along the sagittal plane and sliced after paraffin embedding for HE staining,VG staining,Masson staining,α-SMA immunohistochemistry or CD31 immunohistochemistry.These slices were observed under microscope and the structure of the TM was compared with those of scleral collagen fibers,ciliary muscles and endothelial cells.Results HE staining of the eyeball slices from the 3 animal species resulted in purplish red staining of the TM,which was highly consistent with ciliary muscle fibers. The cell-like structures on the surface of the TM were not clearly outlined, with flat nuclei showing a dark purple staining; these structures did not show obvious boundaries from the TM. Ciliary muscle fibers, which were smooth muscle cells in nature, were aligned in bundles in various directions. The longitudinally sectioned cells were flat and contained purplish cytoplasm and highly flattened nuclei. Scleral collagen fibers were stained dark red with a few fibroblasts sandwiched among them. The long axis of the fibroblasts was in parallel with that of the collagen fibers. The outline of the fibroblast was not clear and the nucleus was flat in dark blue. The vascular endothelial cells presented with different morphologies and contained light purplish cytoplasm and dark nuclei,protruding into the vascular cavity.VG staining of the TM revealed a pale red filamentous structure, and the collagen fibers were stained bright red. Masson staining of the TM showed a reticular structure consisting mainly of dark red fibers intermingled with thin green fibers. Scleral collagen fibers presented with a cord-like green wavy structure. The endothelial cells were green and flat, while the ciliary smooth muscle fibers were purple. In immunohistochemistry for α-SMA, the TM and the ciliary smooth muscle fibers showed a strong positivity in the cytoplasm, while the scleral collagen fibers and vascular endothelial cells showed negative staining;immunohistochemistry for CD31 showed no obvious positive staining in the TM,collagen fibers or ciliary smooth muscle cells from all the animals in spite of slight differences among them. Conclusion The TM consists mainly of smooth muscle fibers with a thin layer of peripheral endomysium without endothelial cells.关键词
小梁网/平滑肌/胶原纤维/内皮细胞/免疫组织化学术/组织切片Key words
trabecular meshwork/smooth muscles/collagen fibers/endothelial cells/immunohistochemistry/tissue slice引用本文复制引用
石赟,周凡琦,罗洲才,陈英华,陈煜,董为人..眼球壁小梁网主要由平滑肌纤维构成且不存在内皮细胞[J].南方医科大学学报,2017,37(10):1400-1404,5.
