南方医科大学学报2017，Vol.37Issue(10)：1405-1409,5.DOI:10.3969/j.issn.1673-4254.2017.10.21
白藜芦醇对老年大鼠骨骼肌力量及肌原纤维结构的影响
Effect of resveratrol on forelimb grip strength and myofibril structure in aged rats
摘要
Abstract
Objective To observe the effect of resveratrol on muscle mass,forelimb grip strength,myofibril structure and AMPK/sirt1 pathway in skeletal muscles of aged rats. Methods Twenty aged (25 months old) SD rats were randomly divided into aged control group and resveratrol treatment group(10 in each group)with 10 young(6 months old)rats served as the young control group. In resveratrol treatment group, the rats were treated with resveratrol (mixed in chow) for 6 weeks.After the treatment, the mass of the gastrocnemius was measured and the sarcopenia index (SI) was calculated as the gastrocnemius mass(mg)to body weight(g)ratio.The forelimb grip strength of the rats was measured using a electronic grip strength meter, and the lengths of the sarcomere, I-band, A-band and H-zone of the myofibrils were determined by transmission electron microscopy.Results Compared with the young rats,the aged control rats had significantly lower SI of the gastrocnemius(P<0.05) and grip strength (P<0.05) with increased lengths of the sarcomere, A-band, I-band and H-zone (P<0.05) and lowered expressions of AMPK, P-AMPK, and sirt1 protein (P<0.05). Resveratrol treatment of the aged rats significantly increased the forelimb grip strength, reduced the lengths of sarcomere length, I-band and H-zone (P<0.05) and increased, P-AMPK, sirt1 protein expressions (P<0.05) without significantly affecting the SI (P>0.05) or the A-band length (P>0.05). Conclusion Resveratrol does not improve the muscle mass but can increase the forelimb grip strength in aged rats possibly by activating AMPK/sirt1 pathway to improve the ultrastructure of the myofibrils.关键词
白藜芦醇/肌肉减少症/抓力/电镜Key words
resveratrol/sarcopenia/grip strength/myofibrils引用本文复制引用
廖芷吟,赵柯湘,肖谦..白藜芦醇对老年大鼠骨骼肌力量及肌原纤维结构的影响[J].南方医科大学学报,2017,37(10):1405-1409,5.基金项目
国家临床重点专科建设项目(国卫办医函[2013]544号) （国卫办医函[2013]544号）
重庆地区老年人群肌肉减少症流行病学调查及危险因素筛查[渝卫科教发(2015MSXM016)] Supported by National Key Clinical Specialties Construction on Program of China(No.[2013]544). （2015MSXM016）
