森林与环境学报2017，Vol.37Issue(4)：392-397,6.DOI:10.13324/j.cnki.jfcf.2017.04.002
杉木愈伤组织及再生芽分化的细胞学观察
Cytohistological observation on callus and regeneration bud differentiation of Cunninghamia lanceolata
摘要
Abstract
To provide scientific basis for the establishment of genetic transformation system of Cunninghamia lanceolata ( Chinese fir) , study on the development of cell tissue in the process of callus formation and regeneration bud differentiation of Chinese fir. The stem segments of Chinese fir were used as explants, analysis of the callus structure, formation and development of cell tissue during the differentiation of regeneration bud were studied with stereomicroscope and paraffin sections. The results showed that callus was first formed from the the epidermal cells split at the incision of stem segments, then gradually formed by the dedifferentiation of vascular parenchyma cells; during the proliferation of callus, meristematic cells would form different vascular tissues; the regeneration bud differentiation of callus was from the epidermal meristem cell of callus, which first forms the bud primordium, and finally formed the visible regenerated bud; adventitious bud can be differentiated from explants directly, or through the incision produced callus. Two kinds of adventitious bud formation way were external origin.关键词
杉木/组织培养/愈伤组织/再生芽/组织细胞学Key words
Cunninghamia lanceolata (Lamb.) Hook./tissue culture/callus/regeneration bud/cytohistological分类
农业科技引用本文复制引用
黄娟,吴鹏飞,魏志超,王玉珍,马祥庆..杉木愈伤组织及再生芽分化的细胞学观察[J].森林与环境学报,2017,37(4):392-397,6.基金项目
国家自然科学基金项目(U1405211) （U1405211）
国家科技支撑计划项目(2015BAD09B010102). （2015BAD09B010102）
