高等学校化学学报2017，Vol.38Issue(10)：1742-1750,9.DOI:10.7503/cjcu20170153
基于超高效液相色谱-四极杆飞行时间质谱的缺血性脑卒中的代谢组学研究
UPLC-QTOF-MS-based Metabolomics Study on Ischemic Stroke Patients
摘要
Abstract
Ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry( UPLC-QTOF-MS) based metabolomics was used to study the metabolism profiling of the plasma from the patients with ischemic stroke, aiming to seek the potential biomarkers of ischemic stroke. 30 patients with ischemic stroke and 17 healthy controls were enrolled. Principal component analysis and orthogonal partial least squares discriminate analysis were used to investigate the metabolic variations between the patients with ischemic stroke and healthy controls. Metabolic pathways were analyzed. Seven biomarkers were obtained. The levels of sphinganine and phytosphingosine were increased, and the levels of glutamine, pyroglutamic acid and 2-keto-butyric acid were decreased. The results of the pathway analysis showed that sphingolipid metabolism and ami-no acid metabolism were perturbed, and the energy metabolism was also disturbed.关键词
代谢组学/超高效液相色谱-四极杆飞行时间质谱/脑卒中/血浆Key words
Metabolomics/Ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry/Ischemic stroke/Plasma分类
化学化工引用本文复制引用
黄玉,谷彩云,吴翰钟,夏晓爽,李新..基于超高效液相色谱-四极杆飞行时间质谱的缺血性脑卒中的代谢组学研究[J].高等学校化学学报,2017,38(10):1742-1750,9.基金项目
国家自然科学基金(批准号:51105274)、 天津市自然科学基金(批准号: 16JCYBJC25500)和天津市科学技术委员会支撑项目(批准号:12ZCZDSY03100)资助.Supported by the National Natural Science Foundation of China ( No. 51105274 ) , the Natural Science Foundation of Tianjin, China ( No.16JCYBJC25500) and the Project of Tianjin Science and Technology Commission, China( No.12ZCZDSY03100) . （批准号:51105274）
