中国实用神经疾病杂志2017，Vol.20Issue(15)：1-5,5.DOI:10.3969/j.issn.1673-5110.2017.15.001
ERK1/2信号通路对缺血后适应大鼠皮层的作用
The effect of ERK1/2 on the ischemic cortex of rats after ischemic postconditioning
摘要
Abstract
Objective To investigate the expression of ERK1/2 in the ischemic cortex of rats after ischemic postconditioning and the effect of extracellular signal-regulated kinase(ERK) inhibitor PD98059 on neuroprotective effects of ischemic postconditioning.and to examine if ischemic postconditioning reduced acute ischemic cerebral infarction followed by reperfusion-induced neurological function and structure injury in rats by increasing the levels of ERK1/2.Methods Twenty (SD) rats were randomly assigned to sham-operated group,ischemic-reperfusion group,ischemic postconditioning group and PD98059+ ischemic postconditioning.We established acute middle cerebral artery occlusion (MCAO) models by using intraluminal suture method.We compared neurological deficit scores in the four groups at 1h and 24h after reperfusion.We compared infarct volume at the end of the 24-hour reperfusion.Furthermore,fifteen rats were added to each group and sacrificed at 2,6 and 24 hours after reperfusion,respectively.The phosphorylation of ERK1/2 and T-ERK1/2 in the ischemic cortex was measured by Western Blot at 2,6,24h after reperfusion.Results Neurological scoring and infarct volume in the group of postconditioning at 2 hours after infarction is minimum among the three groups.But the P-ERK 1/2 in the ischemic cortex of the ischemic postconditioning group were significantly increased at 2,6,24h after reperfusion than other two groups.Conclusion Through the analysis in this experiment,we found that ischemic postconditioning plays a positive role in neuroprotection,and PD98059 could inhibit the expression of ERK in ischemic cortex and decrease the neuroprotective effects,which suggests that MAPK/ERK may participate in neuroprotection of ischemic postconditioning after ischemia/reperfusion injury.关键词
后适应/ERK1/2/PD98059/神经保护/脑缺血Key words
Postconditioning/ERK1/2/PD98059/Neuroprotection/Cerebral ischemia分类
医药卫生引用本文复制引用
谭慧敏,郑兴荣,谭盛..ERK1/2信号通路对缺血后适应大鼠皮层的作用[J].中国实用神经疾病杂志,2017,20(15):1-5,5.基金项目
广州市医药卫生科技项目(20151A011115) （20151A011115）
