中国实用神经疾病杂志2017，Vol.20Issue(15)：6-8,3.DOI:10.3969/j.issn.1673-5110.2017.15.002
氯化钾溶液刺激星形胶质母细胞瘤细胞对MKK4的影响
The effects of KCl stimulation on the expression of MKK4 in glioblastoma cells
摘要
Abstract
Objective To observe the expression of MKK4 and its downstream protein p38 MAPK after stimulating human astrocytoma cells (U87) with different concentrations of KCl,and to explore the expression of MKK4 and p38 MAPK at the level of nucleic acid.Methods U87 cells were stimulated with different concentrations of KCl,the expression of MKK4 and p38 MAPK mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR).The cells were divided into 5 groups:control group (group 0),different concentrations of KC1 group (0.001 mmol/mL,0.025 mmol/mL,0.05 mmol/mL,0.1 mmol/mL,labeled 0.001,0.025,0.05,0.1 group).Results The results of RT-PCR showed that MKK4 and p38 MAPK mRNA were expressed in astrocytes after stimulation with KCl,the expression was increase and then decrease and in the 0.05 group reached the peak,then fall.Conclusion KCl stimulation can increase the expression of MKK4 and p38 MAPK mRNA in human astrocytoma cells,and its expression is related to the concentration of KCl.关键词
氯化钾/星形胶质细胞/MKK4/p38 MAPKKey words
KCl/Astrocyte/MKK4/p38 MAPK分类
医药卫生引用本文复制引用
郝凌云,杨小雪,王沁玥,孙梦兰,潘志强..氯化钾溶液刺激星形胶质母细胞瘤细胞对MKK4的影响[J].中国实用神经疾病杂志,2017,20(15):6-8,3.基金项目
国家自然科学基金面上项目(81671096) （81671096）
江苏省高校自然科学研究面上项目(16KJB320014) （16KJB320014）
徐州医科大学2014年度江苏省重点实验室开放课题(JSBL1404) （JSBL1404）
