华西口腔医学杂志2017，Vol.35Issue(5)：520-526,7.DOI:10.7518/hxkq.2017.05.015
细胞外信号调节蛋白激酶1/2在介导周期性牵张力对牙周膜细胞成骨分化中的作用
The role of extracellular signal regulated kinase 1/2 in mediating osteodifferentiation of human periodontal ligament cells induced by cyclic stretch
摘要
Abstract
Objective This study aimed to investigate the mechanism of cyclic stretch that promotesthe osteogenic diffe-rentiation of human periodontal ligament cells (hPDLCs) through the mediation of extracellular-signal-regulated kinase 1/2 (ERK1/2).Methods hPDLCs were isolated throughthe explant method and culturedin vitro. hPDLCs were mechanically stimulated by a multi-channel cell-stress-loading system for 1, 3, 6, 12, and 24 h. The magnitude of stretch was 10% defor-mation, and the frequency was 0.5 Hz. Nonloaded cells were used as control group. ERK1/2 activation was blocked by U0126, a specific ERK1/2 pathway inhibitor. Additionally, hPDLCs were transfected with adenoviral vector encoding dominant negative ERK1/2 (DN-ERK1/2) to continuouslyinhibit ERK1/2 activation. The mRNA and protein levels of target geneswere detected through real-time polymerase chain reaction and Western blot.Results Cyclic stretching promoted the expression of ERK1/2, osteocalcin (OCN) mRNA, and bone sialoprotein (BSP) mRNA. The expression of runt-related transcription factor (Runx) 2 protein and mRNA also increased at 3 and 6 h of cyclic stretching. The inhibition of ERK1/2 by U0126 and DN-ERK1/2 suppressed the expressionof Runx2 mRNA,OCN mRNA, BSP mRNA, Runx 2 protein, and p-ERK1/2 protein relative to that in stretched cells without the ERK1/2 inhibitor.Conclusion ERK1/2 is a critical molecule in the mediation ofthe osteogenic differentiation of hPDLCs under mechanical stimulation. ERK1/2 activation induced the elevation of Runx2 protein levels, which may be involved in the stretch-induced expressions of OCN and BSP.关键词
牙周膜细胞/成骨分化/周期性牵张力/细胞外信号调节蛋白激酶1/2Key words
periodontal ligament cells/osteogenic differentiation/cyclic stretch/extracellular signal regulated kinase 1/2分类
生物科学引用本文复制引用
宋京,任大鹏,颜世果,蓝菁,袁晓,郭庆圆,戚向敏..细胞外信号调节蛋白激酶1/2在介导周期性牵张力对牙周膜细胞成骨分化中的作用[J].华西口腔医学杂志,2017,35(5):520-526,7.基金项目
国家自然科学基金(31170891) （31170891）
山东省卫生厅重点基金(2011HD001) National Natural Science Foundation of China (31170891) （2011HD001）
Key Fund of Shandong Provincial Health Bureau (2011HD001). （2011HD001）
