海洋科学2017，Vol.41Issue(6)：18-25,8.DOI:10.11759//hykx2016062003
海水青鳉胚胎发育的观察
Observation of embryonic development of marine medaka Oryzias melastigma
摘要
Abstract
This study aims to observe the embryonic development characteristics of marine medaka. As a promising model, Oryzias melastigma was cultivated 30 days after hatching as broodstock and naturally fertilized eggs were collected. The fertilized eggs were incubated in filtered sea water at 26–28℃ and at 32–33 salinity without ventila-tion. The ontogenetic embryos of O. melastigma were observed using an Olympus SZ-61 microscope. The total development time of every stage was noted and calculated; characteristic images were taken using a digital camera. The results show that the fertilized eggs were globular, multiple oil globules, or demersal. The outermost structure of the egg was covered with a number of short villi and long attaching filaments. The embryonic development in-cludes various stages such as the blastodisc, cleavage, blastula, gastrula, neurula, organogenesis, tail bud, heart beating, and hatching. Significant differences between O. melastigma were observed, which are as follows: (1) Irregular blastomeres occurred in early cleavage; (2) The way of hatching was tail-first escaping from the chorion; (3) Two Kupffer, s vesicles appeared in some embryos.关键词
海水青鳉(Oryzias melastigma)/胚胎发育/柯氏囊Key words
marine medaka (Oryzias melastigma)/embryonic development/Kupffer/s vesicle分类
农业科技引用本文复制引用
王友红,刘洪军,于道德,李玉全,官曙光,刘莹..海水青鳉胚胎发育的观察[J].海洋科学,2017,41(6):18-25,8.基金项目
National Natural Science Foundation of China, No.31101916 （）
Agricultural Seeds Engineering Projects of Shandong, No.2014-2016 （）
Peninsula Nearshore Ecosystem Simulation Experiment Research Projects of Shandong国家自然科学基金项目(31101916) （31101916）
山东省农业良种工程项目(2014-2016) （2014-2016）
山东半岛近岸海域生态模拟实验研究项目 （）
