海洋科学2017，Vol.41Issue(6)：41-47,7.DOI:10.11759/hykx20151224003
三疣梭子蟹(Portunus trituberculatus)冻藏过程中肌肉蛋白质生化特性的变化
Changes in the biochemical properties of muscle protein of swimming crab (Portunus trituberculatus) during frozen storage
摘要
Abstract
This study analyzed the changes in the biochemical characteristics of muscle protein during frozen stor-age at –20℃ or –40℃ based on nutrient composition, protein composition, myofibrillar protein content, Ca2+-ATPase ac-tivity, sulfhydryl group content, disulfide bond content, and SDS-PAGE analysis of myofibrillar protein of swim-ming crab. Results showed that the muscle of the swimming crab was a typical food with a high protein content. With extension of the duration of frozen storage, the water-soluble protein content increased and then decreased, the salt-soluble protein and -insoluble protein contents decreased, and the content of alkaline-soluble protein increased gradually. Myofibrillar protein content, Ca2+-ATPase activity, and sulfhydryl group content decreased with prolon-gation of the duration of frozen storage, whereas the disulfide bond content showed an increasing trend, with a sig-nificant difference between the two groups at –20℃ and –40℃ (p < 0.05). The SDS-PAGE results showed that all types of proteins of myofibrillar protein were degraded to different degrees, and the degradation was more apparent at ?20℃ than at ?40℃. Therefore, frozen storage at –40℃ has minimal effect on the biochemical characteristics of muscle protein.关键词
三疣梭子蟹(Portunustrituberculatus)/肌肉/蛋白质/生化特性Key words
swimming crab (Portunus trituberculatus)/muscle/protein/biochemical properties分类
轻工纺织引用本文复制引用
黄琳,张莉,武丹露..三疣梭子蟹(Portunus trituberculatus)冻藏过程中肌肉蛋白质生化特性的变化[J].海洋科学,2017,41(6):41-47,7.基金项目
Province Natural Science Foundation of Shandong, NO.ZR2015CM010山东省自然科学基金项目(ZR2015CM010) （ZR2015CM010）
