华中师范大学学报（自然科学版）2017，Vol.51Issue(5)：646-650,5.DOI:10.19603/j.cnki.1000-1190.2017.05.015
氯胺酮相关性膀胱炎大鼠逼尿肌热休克蛋白27的表达及其意义
Expressions and significances of heat shock protein 27 in detrusor tissue of rats with ketamine-associated cystitis
摘要
Abstract
To investigate the expression of heat shock protein 27 (HSP27) in detrusor tissue of rats with ketamine and clinical significance.Rats were injected intraperitoneal with ketamine to establish the cystitis model.Through urodynamic detection the function of bladder contraction was studied,and then urinary bladders of the rats were harvested for routine pathological staining to analysis bladder mucosa and detrusor structure.HSP27 expression level in detrusor tissue at different time point was detected by immunohistochemical (IHC) staining.In comparison to the control group,urodynamic change of ketamine treated group showed significantly increased frequency of bladder contraction and reduced bladder capacity of first sensation;there is no significant change on pathological and immune expression in the control group,while it was observed in the experimental group with different degree of bladder mucosal hyperemia,inflammatory cells infiltration and elevated HSP27 expression levels;additionally,HSP27 expression level was positively correlated with the induction time.These results suggested that the expression levels of HSP27 might have a correlation with the degree of detrusor contraction activities of ketamine-associated cystitis.关键词
氯胺酮/热休克蛋白27/大鼠Key words
ketamine/heat shock protein 27/rats分类
医药卫生引用本文复制引用
林黎,王智超,朱保平..氯胺酮相关性膀胱炎大鼠逼尿肌热休克蛋白27的表达及其意义[J].华中师范大学学报（自然科学版）,2017,51(5):646-650,5.基金项目
湖北省自然科学基金项目(2012FFA094). （2012FFA094）
