家畜生态学报2017，Vol.38Issue(9)：13-20,8.
山羊VPS13C和EIF4G1基因多态性及其与产奶性状关联性分析
Identification of SNPs in VPS13C and EIF4G1 and their Association with Milk Production in Goat
摘要
Abstract
In order to analyze the association between polymorphisms of the VPS13C and EIF4G1 gene and milk traits of dairy goat,Toggenburg and Laoshan dairy goats were selected as the experimental group,and Liaoning,Ritu,Bange,Nanjiang cashmere goats were used as the control group.In this study,polymorphisms of exon 10,33 and intron 10 of VPS13C and exon 18,24 of EIF4G1 were detected by DNA sequencing method.The results showed that,one and three polymorphic loci were detected in exon 10,33 and intron 10 of VPS13C gene respectively.Among them,g.22885C＞A and g.72809G＞A caused the changes in amino acids.Association analysis showed that there were no significant correlation between the different genotypes of the five polymorphic loci and the average daily milk yield of Laoshan dairy goats.However,two common haplotypes were identified in all studied goat populations.Haplotype ACAG in LD block was dominant in dairy goat (63.7％) and segregated at higher frequency than that in non-dairy goat (21.2％),indicating that the association of ACAG haplotype to the milk production performance of dairy goats.In addition,two polymorphic loci were found in exon 18 and 24 of EIF4G1 gene.Mutation g.12301A＞G resulted in amino acid change,and mutation g.9003G＞A did not cause any amino acid change.In Laoshan dairy goat,g.9003G＞A assumed two genotypes named AA and AG respectively.The association analysis of genotypes and average daily milk yield revealed that individuals with genotype AA were significantly higher than those with genotype AG(P=0.021).These findings suggested that VPS13C and EIF4G1 gene could be the candidate genes of milk traits in dairy goat,and the g.9003G＞A loci of EIF4G1 might be an effective molecular marker for milk performance of dairy goat.关键词
奶山羊/VPS13C基因/EIF4G1基因/产奶性状Key words
dairy goat/VPS13C gene/EIF4G1 gene/milk traits分类
农业科技引用本文复制引用
玛哈巴·肉孜,宋伸,潘建飞,杨敏,孟详人,赵金山,蒋琳,马月辉..山羊VPS13C和EIF4G1基因多态性及其与产奶性状关联性分析[J].家畜生态学报,2017,38(9):13-20,8.基金项目
国家自然基金(31272403,31472064,31601910) （31272403,31472064,31601910）
现代农业产业技术体系(CARS-40-01) （CARS-40-01）
公益性行业(农业)科研专项(201303059) （农业）
中央级公益性科研院所基本科研业务费(2015ywf-zd-1) （2015ywf-zd-1）
