家畜生态学报2017，Vol.38Issue(9)：21-24,4.
家驴与藏野驴SRY基因多态性研究
Polymorphism of SRY Gene between Equus kiang and Equus asinus
王西峰 1雷初朝 2晁生玉 1乌兰巴特尔 1王照忠 1王成林 1王启菊 1赵森 1马志杰 3党瑞华2
作者信息
- 1. 青海省海西州动物疫病预防控制中心,青海德令哈817099
- 2. 西北农林科技大学动物科技学院,陕西杨凌712100
- 3. 青海省畜牧兽医科学院,青海西宁810016
- 折叠
摘要
Abstract
The objective of this study is to amplify SRY gene in Equus kiang and explore the paternal genetic relationship among the genus Equus to provide a theoretical foundation for conservation of germplasm resources in Equus kiang.The genomic DNA of ear tissue or hair of two Equus kiang and two Qinghai Equus asinus was extracted,and then was used to amplify and sequence 1038 bp fragment of SRY gene,which consists of 5'-noncoding region of 330 bp and the whole coding region of 708 bp.The result of sequence alignment showed that there were four differences of SRY gene between Qinghai Equus asinus and Equuskiang (-265G＞C/G,-131T＞C/T,4933G＞A,629A＞C),the latter two of which lead to change of amino acid (Thr 165 Ala,Thr 210 Lys).The result of phylogenetic tree showed that paternal genetic relationship in the genus Equus was that Equus asinus firstly converged with Equus kiang,secondly converged with Equus burchellii,Equus grevyi and Equus zebra,lastly converged with Equus caballus,indicating the close relationship between Equus kiang and Equus asinus.关键词
家驴/藏野驴/SRY基因/多态性Key words
Equus asinu/Equus kiang/SRY gene/polymorphism分类
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王西峰,雷初朝,晁生玉,乌兰巴特尔,王照忠,王成林,王启菊,赵森,马志杰,党瑞华..家驴与藏野驴SRY基因多态性研究[J].家畜生态学报,2017,38(9):21-24,4.
