解剖学杂志2017，Vol.40Issue(5)：529-534,6.DOI:10.3969/j.issn.1001-1633.2017.05.008
云南大理白族地区乳腺癌EphA2和Ephrin A1的表达及其意义
Expression and clinical significance of EphA2 and Ephrin A1 in the breast cancer tissue of females from Bai ethnic group of Dali in Yunnan province
摘要
Abstract
Objective:To study the expression of EphA2 and EphrinA1 in the breast cancer tissue of females from Bai ethnic group of Dali and to explore their clinical significance.Methods:Totally 150 paraffin specimens of breast cancer were selected from the affiliated hospital of Dali University from the year 2012 to 2015.The expression of EphA2 and Ephrin A1 in the breast cancer tissues was examined by immunohistochemistry and in situ hybridization, and their relationships with clinocopathological parameters and with each other were analyzed.Results:The protein and mRNA of EphA2 and Ephrin A1 were mainly expressed in cytoplasm and membrane of tumor cells and vascular endothelial cells.The positive rates of EphA2 and Ephrin A1 expression were 82% and 86%,respectively;while the positive rates of their mRNA expression were 60.67%and 65 .33%,respectively.The positive expression rates regarding the protein and mRNA of EphA2 and Ephrin A1 had no significant correlation with patient age,but were correlated with the pathological type,tumor size,lymph node metastasis, clinical stage and histological grade statistically.The positive rates of the protein and mRNA of EphA2 and Ephrin A1 in the invasive ductal carcinoma were higher than that in the intraductal carcinoma;and the positive rates of the protein and mRNA of EphA2 and Ephrin A1 in the group with large size,lymph node metastasis,late clinical stage and with high histological grade were higher than that in the group with small size,negative lymph node metastasis,early clinical stage and with low histological grade,respectively.The positive protein staining of EphA2 and Ephrin A1 were co-located in roughly the same tumor areas and vascular endothelial cells,and their distributions were fundamentally unanimous and their positive expression rates were positively correlated;and the positive mRNA staining of EphA2 and Ephrin A1 had a consistent distribution pattern to that of the protein staining,and their positive rates were also positively correlated.The positive expression rates aiming at the protein of EphA2 and Ephrin A1 were related to that aiming at their corresponding mRNA,but with no identical expression level.Conclusion:The over-expression of EphA2 and Ephrin A1 is present in breast cancer,and is involved in carcinogenesis,invasion, metastasis and malignant transformation, which reveals that EphA2 and Ephrin A1 are expected to become valuable new markers for the early diagnosis and prognosis evaluation,and therefore they could be attractive new targets for gene therapy of breast cancer.关键词
白族/乳腺癌/EphA2/EphrinA1Key words
Bai ethnicity/breast cancer/EphA2/Ephrin A1引用本文复制引用
张本斯,李庄,杨桂,张秀君,唐洗敏,荆永光,徐国萍..云南大理白族地区乳腺癌EphA2和Ephrin A1的表达及其意义[J].解剖学杂志,2017,40(5):529-534,6.基金项目
国家自然科学基金(81460465) （81460465）
