江苏大学学报（医学版）2017，Vol.27Issue(5)：391-396,6.DOI:10.13312/j.issn.1671-7783.y170088
miR-187在胃癌中的表达及其对胃癌细胞增殖和侵袭的影响
Expression of microRNA-187 in gastric carcinoma and its effect on proliferation and invasion of gastric cancer cells
摘要
Abstract
Objective:To investigate the effect of abnormal expression of microRNA-187 (miR-187) on proliferation,invasion and migratory of gastric cancer.Methods:A total of 24 pairs of human gastric cancer tumor and matched non-tumor tissues were collected and miR-187 expression was detected and their relevance was analyzed.The expression of miR-187 and CD276 in human gastric cancer cell SGC-7901 were inhibited by siRNA transfection,and then the effect of SGC-7901 cell proliferation and invasion-migration was detected by CCK-8 and Transwell assay respectively.The expression of matrix metalloproteinase-9 (MMP-9) was detected by Western blotting.Results:Compared with matched non-tumor tissues,the expression of miR-187 was increased in stage Ⅰ,Ⅱ,Ⅲ and Ⅳ gastric cancer(P ＜0.01);the expression of CD276 was decreased in stage Ⅰ,Ⅱ,Ⅲ and Ⅳgastric cancer(P ＜0.01).The correlation coefficient of miR-187 expression and CD276 expression in gastric carcinoma was 0.840.Compared with group miR-187 inhibitor-c,the expression of CD276 was up-regulated and MMP-9 was down-regulated(P ＜0.01),the proliferation of SGC-7901 and the invasion and migration of SGC-7901 was inhibited (P ＜ 0.01) in group miR-187 inhibitor.Compared with group siRNA-negative control,the expression of MMP-9 was up-regulated in the group CD276-siRNA (P ＜0.01).Conclusion:MicroRNA-187 was increased in gastric carcinoma and the proliferation and invasion of human gastric cancer cell was promoted by miR-187.关键词
miR-187/胃癌/增殖/侵袭/迁移Key words
microRNA-187/gastric cancer/invasion/growth/migration分类
医药卫生引用本文复制引用
王馨,苏兆亮,许文林,沈慧玲..miR-187在胃癌中的表达及其对胃癌细胞增殖和侵袭的影响[J].江苏大学学报（医学版）,2017,27(5):391-396,6.基金项目
国家自然科学基金资助项目(81101677,81672913) （81101677,81672913）
江苏省自然科学基金资助项目(BK20141288) （BK20141288）
