实用临床医药杂志2017，Vol.21Issue(17)：64-68,5.DOI:10.7619/jcmp.201717019
益气养阴通络方对IgA肾病模型大鼠肾脏组织肿瘤坏死因子-α的影响
Effect of yiqi yangyin tongluo regime on expression of TNF-α in renal tissue in rats with IgA nephropathy
摘要
Abstract
Objective To explore the effect of yiqi yangyin tongluo regime on hematuria,proteinuria and renal tissue's pathological changes in rats with IgA nephropathy (IgAN).Methods Sixty clean male SD rats were randomized into blank group,model group,huangkui group and high,medium and low-dose TCM (yiqi yangyin tongluo regime) group.After modeling for 0 week,4 weeks,8 weeks,12 weeks and 16 weeks,the levels of urine red blood cell count and 24 h urine protein were detected.Meanwhile,after feeding for 8 weeks,the pathological changes of remnant renal tissues and expression of TNF-α were detected by HE staining and immunohistochemical staining.Results After feeding for 8 weeks,compared with blank group,the level of urine red blood cell count,24h urine protein and TNF-α expression in model group,huangkui group,high,medium and low-dose TCM group were significantly higher (P ＜ 0.05).Compared with the model group,the level of urine red blood cell count,24h urine protein and TNF-α expression in huangkui group,high,medium and low-dose TCM group were significantly lower (P ＜ 0.05).The above indexes in TCM group were significantly lower than huangkui group (P ＜ 0.05).Light microscopy showed that the renal tissues were normal in blank group.In the model group,most of glomerular mesangial cells and matrix appeared moderate and severe hyperplasia,inflammatory ceils and focal fibrosis were seed in renal interstitium.Proliferation of mesangial cells and matrix were relieved in huangkui group and TCM group.Conclusion Yiqi yangyin tongluo regime can effectively ameliorate the levels of urine red blood cell count,24 h urine protein and renal pathological changes.The mechanism may be related to the decrease of urine red blood cell count and 24 h urinary protein,and inhibition of TNF-α expression.关键词
IgA肾病/益气养阴通络方/机制/TNF-αKey words
IgA nephropathy/yiqi yangyin tongluo regime/mechanism/TNF-α分类
医药卫生引用本文复制引用
强胜,杜珍芳,黄敏,马晓伟,周奕岑..益气养阴通络方对IgA肾病模型大鼠肾脏组织肿瘤坏死因子-α的影响[J].实用临床医药杂志,2017,21(17):64-68,5.基金项目
江苏省苏州市科技局基金项目(SYSD2013156) （SYSD2013156）
